A G-protein β-subunit is essential for Dictyostelium development

Pamela Lilly, Lijun Wu, Dennis L. Welker, Peter N Devreotes

Research output: Contribution to journalArticle

Abstract

Recent studies have demonstrated that G-protein-linked signal transduction pathways play a significant role in the developmental program of the simple eukaryotic organism Dictyostelium. We have reported previously the isolation of a G-protein β-subunit and present here a more complete analysis of this gene. Low-stringency Southern blots and RFLP mapping studies suggest that the β-subunit is a unique gene found on linkage group II. Its deduced amino acid sequence of 347 residues is ∼60% identical to those of the human, Drosophila, and Caenorhabditis elegans β-subunits. The carboxy-terminal 300 residues are about 70% identical; the amino-terminal 50 residues are quite divergent, containing only 10 identities. At all stages of growth and development, a single 1.9-kb β-subunit mRNA is present at a high level, and a specific antibody detects a single 37-kD protein. We propose that G-protein heterotrimers are formed when this β-subunit couples with each of the eight distinct G-protein α-subunits that are transiently expressed during development. Targeted disruption of the β-subunit gene had no effect on the viability of haploid cells, but resulted in the inability of cells to aggregate.

Original languageEnglish (US)
Pages (from-to)986-995
Number of pages10
JournalGenes & development
Volume7
Issue number6
StatePublished - Jun 1993

Fingerprint

Dictyostelium
Protein Subunits
GTP-Binding Proteins
Genes
Haploidy
Caenorhabditis elegans
Southern Blotting
Growth and Development
Restriction Fragment Length Polymorphisms
Drosophila
Amino Acid Sequence
Signal Transduction
Messenger RNA
Antibodies
Proteins

Keywords

  • Chemotaxis
  • Gene targeting
  • Heterotrimeric G-proteins
  • Signal transduction

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

A G-protein β-subunit is essential for Dictyostelium development. / Lilly, Pamela; Wu, Lijun; Welker, Dennis L.; Devreotes, Peter N.

In: Genes & development, Vol. 7, No. 6, 06.1993, p. 986-995.

Research output: Contribution to journalArticle

Lilly, P, Wu, L, Welker, DL & Devreotes, PN 1993, 'A G-protein β-subunit is essential for Dictyostelium development', Genes & development, vol. 7, no. 6, pp. 986-995.
Lilly, Pamela ; Wu, Lijun ; Welker, Dennis L. ; Devreotes, Peter N. / A G-protein β-subunit is essential for Dictyostelium development. In: Genes & development. 1993 ; Vol. 7, No. 6. pp. 986-995.
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