A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: First molecular traceback to deli sandwiches contaminated during preparation

Nicholas A. Daniels, David A. Bergmire-Sweat, Kellogg Schwab, Kate A. Hendricks, Sudha Reddy, Steven M. Rowe, Rebecca L. Fankhauser, Stephan S. Monroe, Robert L. Atmar, Roger I. Glass, Paul Mead

Research output: Contribution to journalArticle

Abstract

In March 1998, an outbreak of acute gastroenteritis occurred among students at a Texas university. Overall, 125 ill students sought medical care. Case-control studies revealed that illness was significantly associated with eating foods from the university's main cafeteria dell bar on 9 and 10 March. Stool specimens from 9 (50%) of 18 ill students and samples of deli ham showed evidence of Norwalk-like viruses (NLVs) by reverse-transcriptase (RT) polymerase chain reaction (PCR) assay. A food handler who prepared sandwiches for lunch on 9 March reported that her infant had been sick with watery diarrhea since just before the outbreak. A stool sample from the infant was positive for NLV by RT-PCR, and the sequence of the amplified product was identical to that of amplified product from dell ham and students' stool specimens. This is the first time RT-PCR and sequence analysis have successfully confirmed vital contamination of a food item likely to have been contaminated by a food handler.

Original languageEnglish (US)
Pages (from-to)1467-1470
Number of pages4
JournalJournal of Infectious Diseases
Volume181
Issue number4
DOIs
StatePublished - 2000

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Norovirus
Gastroenteritis
Reverse Transcriptase Polymerase Chain Reaction
Disease Outbreaks
Students
Food
Food Contamination
Lunch
Medical Students
Sequence Analysis
Case-Control Studies
Diarrhea
Eating

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses : First molecular traceback to deli sandwiches contaminated during preparation. / Daniels, Nicholas A.; Bergmire-Sweat, David A.; Schwab, Kellogg; Hendricks, Kate A.; Reddy, Sudha; Rowe, Steven M.; Fankhauser, Rebecca L.; Monroe, Stephan S.; Atmar, Robert L.; Glass, Roger I.; Mead, Paul.

In: Journal of Infectious Diseases, Vol. 181, No. 4, 2000, p. 1467-1470.

Research output: Contribution to journalArticle

Daniels, NA, Bergmire-Sweat, DA, Schwab, K, Hendricks, KA, Reddy, S, Rowe, SM, Fankhauser, RL, Monroe, SS, Atmar, RL, Glass, RI & Mead, P 2000, 'A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses: First molecular traceback to deli sandwiches contaminated during preparation', Journal of Infectious Diseases, vol. 181, no. 4, pp. 1467-1470. https://doi.org/10.1086/315365
Daniels, Nicholas A. ; Bergmire-Sweat, David A. ; Schwab, Kellogg ; Hendricks, Kate A. ; Reddy, Sudha ; Rowe, Steven M. ; Fankhauser, Rebecca L. ; Monroe, Stephan S. ; Atmar, Robert L. ; Glass, Roger I. ; Mead, Paul. / A foodborne outbreak of gastroenteritis associated with Norwalk-like viruses : First molecular traceback to deli sandwiches contaminated during preparation. In: Journal of Infectious Diseases. 2000 ; Vol. 181, No. 4. pp. 1467-1470.
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