A flow-through tissue culture system with fast dynamic response

J. Lankelma, E. Laurensse, H. M. Pinedo

Research output: Contribution to journalArticlepeer-review

Abstract

A flow-through system in which monolayer cells, growing on a 50-cm2 glass surface, are in contact with a film of medium with a thickness of 0.14 mm, is described. For murine B16 melanoma cells, the loading capacity is 8.106 cells. The flow-through principle permits frequent off-line or on-line detection of medium constituents for a period on the order of days. The system has a fast dynamic response. With off-line radiochemical detection, the system was applied to the uptake of uridine and excretion of uracil over a period of 45 h. With on-line fluorescence detection, the interaction between the cells and two anthracycline analogs was monitored. The cells can be easily observed with a light microscope.

Original languageEnglish (US)
Pages (from-to)340-345
Number of pages6
JournalAnalytical Biochemistry
Volume127
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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