A flow cytometric crossmatch test using endothelial precursor cells isolated from peripheral blood

Annette M. Jackson, Donna P. Lucas, Jessica L. Badders

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.

Original languageEnglish (US)
Title of host publicationTransplantation Immunology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages319-329
Number of pages11
ISBN (Print)9781627034920
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1034
ISSN (Print)1064-3745

Keywords

  • Alloantibodies
  • Endothelial cell
  • Flow cytometric crossmatch
  • Non-HLA antibodies
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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