A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: A novel defect in plasmalogen biosynthesis

Narasimhan Nagan, Amiya K. Hajra, Arun K. Das, Hugo W. Moser, Ann Moser, Paul Lazarow, P. Edward Purdue, Raphael A. Zoeller

Research output: Contribution to journalArticle

Abstract

Using fluorescence-activated cytotoxicity selection, followed by colony autoradiographic screening of the surviving population, we have isolated a unique plasmalogendeficient Chinese hamster ovary (CHO) cell line. The mutant, NZel-1, showed a dramatic (90%) reduction in the rate of biosynthesis and levels of plasmalogens, as determined using short- and long-term labeling with 32P(i). Enzymatic assays and lipid supplementation studies showed that NZel-1 was defective in a single step in the biosynthetic pathway for plasmalogens. This step, catalyzed by the peroxisomal enzyme, alkyldihydroxyacetone phosphate (DHAP) synthase, is responsible for the introduction of the ether bond found in plasmalogens. The activity of alkyl- DHAP synthase was reduced in whole-cell homogenates from NZel-1 to 18% of wild-type values. Unlike previously described plasmalogen-deficient mutants, NZel-1 contained peroxisomes, as confirmed by immunofluorescence microscopy and catalase release by digitonin. Peroxisomal functions, including the breakdown of very long-chain (>20 carbons) fatty acids, phytanic acid oxidation, and the acylation of DHAP, were normal. Cell fusion studies revealed that the mutation is recessive and belongs to a new complementation group. To our knowledge this is the first report describing the isolation and characterization of a mutant CHO cell line defective in plasmalogen biosynthesis which contains intact, functional peroxisomes. These cells will allow us to examine the role of ether lipids in cellular functions without complications associated with peroxisome deficiency.

Original languageEnglish (US)
Pages (from-to)4475-4480
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number9
DOIs
StatePublished - Apr 29 1997

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'A fibroblast cell line defective in alkyl-dihydroxyacetone phosphate synthase: A novel defect in plasmalogen biosynthesis'. Together they form a unique fingerprint.

Cite this