TY - JOUR
T1 - A facile method for the labeling of proteins with zirconium isotopes
AU - Meijs, Wilma E.
AU - Haisma, Hidde J.
AU - Van Der Schors, Roel
AU - Wijbrandts, René
AU - Van Den Oever, Karin D.
AU - Klok, Rob P.
AU - Pinedo, Herbert M.
AU - Herscheid, Jacobus D M
PY - 1996/5
Y1 - 1996/5
N2 - ABSTRACT. To label proteins with positron emitters with a half-life in the order of days, a method has been developed to label proteins with zirconium (Zr)-isotopes. Therefore, the bifunctional chelating agent desferal (Df) was coupled to albumins via a thioether bond. Labeling of the premodified proteins was easily performed by addition of these proteins to freeze dried Zr oxalate, This labeling was efficient (>90%) and accomplished in several minutes. The conjugates showed a high in vitro stability. Biodistribution studies were performed with 88Zr-citrate, 88Zr-Df, and 88Zr-labeled mouse serum albumin (88Zr-Df-MSA), modified with different amounts of chelating groups. Whereas Zr-citrate was found to accumulate in bone, Zr-Df was cleared very fast by glomerular filtration. The 88Zr-Df-MSA showed similar blood clearance as did 123I-labeled MSA. The biodistribution pattern of 88Zr-Df-MSA differed only from 123I-MSA in that a higher accumulation of Zr in liver, kidney, and spleen was found. The absence of large amounts of 88Zr in bone indicated that in vivo the conjugates are also reasonably stable.
AB - ABSTRACT. To label proteins with positron emitters with a half-life in the order of days, a method has been developed to label proteins with zirconium (Zr)-isotopes. Therefore, the bifunctional chelating agent desferal (Df) was coupled to albumins via a thioether bond. Labeling of the premodified proteins was easily performed by addition of these proteins to freeze dried Zr oxalate, This labeling was efficient (>90%) and accomplished in several minutes. The conjugates showed a high in vitro stability. Biodistribution studies were performed with 88Zr-citrate, 88Zr-Df, and 88Zr-labeled mouse serum albumin (88Zr-Df-MSA), modified with different amounts of chelating groups. Whereas Zr-citrate was found to accumulate in bone, Zr-Df was cleared very fast by glomerular filtration. The 88Zr-Df-MSA showed similar blood clearance as did 123I-labeled MSA. The biodistribution pattern of 88Zr-Df-MSA differed only from 123I-MSA in that a higher accumulation of Zr in liver, kidney, and spleen was found. The absence of large amounts of 88Zr in bone indicated that in vivo the conjugates are also reasonably stable.
KW - Desferal
KW - Protein labeling
KW - Zirconium-88
KW - Zirconium-89
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U2 - 10.1016/0969-8051(96)00020-0
DO - 10.1016/0969-8051(96)00020-0
M3 - Article
C2 - 8832698
AN - SCOPUS:0030152944
SN - 0969-8051
VL - 23
SP - 439
EP - 448
JO - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
JF - International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
IS - 4
ER -