A facile method for the labeling of proteins with zirconium isotopes

Wilma E. Meijs, Hidde J. Haisma, Roel Van Der Schors, René Wijbrandts, Karin D. Van Den Oever, Rob P. Klok, Herbert M. Pinedo, Jacobus D M Herscheid

Research output: Contribution to journalArticlepeer-review

Abstract

ABSTRACT. To label proteins with positron emitters with a half-life in the order of days, a method has been developed to label proteins with zirconium (Zr)-isotopes. Therefore, the bifunctional chelating agent desferal (Df) was coupled to albumins via a thioether bond. Labeling of the premodified proteins was easily performed by addition of these proteins to freeze dried Zr oxalate, This labeling was efficient (>90%) and accomplished in several minutes. The conjugates showed a high in vitro stability. Biodistribution studies were performed with 88Zr-citrate, 88Zr-Df, and 88Zr-labeled mouse serum albumin (88Zr-Df-MSA), modified with different amounts of chelating groups. Whereas Zr-citrate was found to accumulate in bone, Zr-Df was cleared very fast by glomerular filtration. The 88Zr-Df-MSA showed similar blood clearance as did 123I-labeled MSA. The biodistribution pattern of 88Zr-Df-MSA differed only from 123I-MSA in that a higher accumulation of Zr in liver, kidney, and spleen was found. The absence of large amounts of 88Zr in bone indicated that in vivo the conjugates are also reasonably stable.

Original languageEnglish (US)
Pages (from-to)439-448
Number of pages10
JournalNuclear Medicine and Biology
Volume23
Issue number4
DOIs
StatePublished - May 1996
Externally publishedYes

Keywords

  • Desferal
  • Protein labeling
  • Zirconium-88
  • Zirconium-89

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

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