A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment

Stefan Scholz, Erika Sela, Ludek Blaha, Thomas Braunbeck, Malyka Galay-Burgos, Mauricio García-Franco, Joaquin Guinea, Nils Klüver, Kristin Schirmer, Katrin Tanneberger, Marysia Tobor-Kapłon, Hilda Witters, Scott Belanger, Emilio Benfenati, Stuart Creton, Mark T D Cronin, Rik I L Eggen, Michelle Embry, Drew Ekman, Anne Gourmelon & 23 others Marlies Halder, Barry Hardy, Thomas Hartung, Bruno Hubesch, Dirk Jungmann, Mark A. Lampi, Lucy Lee, Marc Léonard, Eberhard Küster, Adam Lillicrap, Till Luckenbach, Albertinka J. Murk, José M. Navas, Willie Peijnenburg, Guillermo Repetto, Edward Salinas, Gerrit Schüürmann, Horst Spielmann, Knut Erik Tollefsen, Susanne Walter-Rohde, Graham Whale, James R. Wheeler, Matthew J. Winter

Research output: Contribution to journalArticle

Abstract

Tests with vertebrates are an integral part of environmental hazard identification and risk assessment of chemicals, plant protection products, pharmaceuticals, biocides, feed additives and effluents. These tests raise ethical and economic concerns and are considered as inappropriate for assessing all of the substances and effluents that require regulatory testing. Hence, there is a strong demand for replacement, reduction and refinement strategies and methods. However, until now alternative approaches have only rarely been used in regulatory settings. This review provides an overview on current regulations of chemicals and the requirements for animal tests in environmental hazard and risk assessment. It aims to highlight the potential areas for alternative approaches in environmental hazard identification and risk assessment. Perspectives and limitations of alternative approaches to animal tests using vertebrates in environmental toxicology, i.e. mainly fish and amphibians, are discussed. Free access to existing (proprietary) animal test data, availability of validated alternative methods and a practical implementation of conceptual approaches such as the Adverse Outcome Pathways and Integrated Testing Strategies were identified as major requirements towards the successful development and implementation of alternative approaches. Although this article focusses on European regulations, its considerations and conclusions are of global relevance.

Original languageEnglish (US)
Pages (from-to)506-530
Number of pages25
JournalRegulatory Toxicology and Pharmacology
Volume67
Issue number3
DOIs
StatePublished - Dec 2013

Fingerprint

Animal Testing Alternatives
Risk assessment
Hazards
Animals
Vertebrates
Effluents
Testing
Ecotoxicology
Disinfectants
Chemical plants
Amphibians
Fish
Fishes
Economics
Availability
Pharmaceutical Preparations

Keywords

  • 3Rs
  • Acute toxicity
  • Bioconcentration
  • Cell lines
  • Chronic toxicity
  • Endocrine disruption
  • Exposure-based waiving
  • Fish embryo test
  • QSAR
  • Read-across

ASJC Scopus subject areas

  • Toxicology

Cite this

Scholz, S., Sela, E., Blaha, L., Braunbeck, T., Galay-Burgos, M., García-Franco, M., ... Winter, M. J. (2013). A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment. Regulatory Toxicology and Pharmacology, 67(3), 506-530. https://doi.org/10.1016/j.yrtph.2013.10.003

A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment. / Scholz, Stefan; Sela, Erika; Blaha, Ludek; Braunbeck, Thomas; Galay-Burgos, Malyka; García-Franco, Mauricio; Guinea, Joaquin; Klüver, Nils; Schirmer, Kristin; Tanneberger, Katrin; Tobor-Kapłon, Marysia; Witters, Hilda; Belanger, Scott; Benfenati, Emilio; Creton, Stuart; Cronin, Mark T D; Eggen, Rik I L; Embry, Michelle; Ekman, Drew; Gourmelon, Anne; Halder, Marlies; Hardy, Barry; Hartung, Thomas; Hubesch, Bruno; Jungmann, Dirk; Lampi, Mark A.; Lee, Lucy; Léonard, Marc; Küster, Eberhard; Lillicrap, Adam; Luckenbach, Till; Murk, Albertinka J.; Navas, José M.; Peijnenburg, Willie; Repetto, Guillermo; Salinas, Edward; Schüürmann, Gerrit; Spielmann, Horst; Tollefsen, Knut Erik; Walter-Rohde, Susanne; Whale, Graham; Wheeler, James R.; Winter, Matthew J.

In: Regulatory Toxicology and Pharmacology, Vol. 67, No. 3, 12.2013, p. 506-530.

Research output: Contribution to journalArticle

Scholz, S, Sela, E, Blaha, L, Braunbeck, T, Galay-Burgos, M, García-Franco, M, Guinea, J, Klüver, N, Schirmer, K, Tanneberger, K, Tobor-Kapłon, M, Witters, H, Belanger, S, Benfenati, E, Creton, S, Cronin, MTD, Eggen, RIL, Embry, M, Ekman, D, Gourmelon, A, Halder, M, Hardy, B, Hartung, T, Hubesch, B, Jungmann, D, Lampi, MA, Lee, L, Léonard, M, Küster, E, Lillicrap, A, Luckenbach, T, Murk, AJ, Navas, JM, Peijnenburg, W, Repetto, G, Salinas, E, Schüürmann, G, Spielmann, H, Tollefsen, KE, Walter-Rohde, S, Whale, G, Wheeler, JR & Winter, MJ 2013, 'A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment', Regulatory Toxicology and Pharmacology, vol. 67, no. 3, pp. 506-530. https://doi.org/10.1016/j.yrtph.2013.10.003
Scholz, Stefan ; Sela, Erika ; Blaha, Ludek ; Braunbeck, Thomas ; Galay-Burgos, Malyka ; García-Franco, Mauricio ; Guinea, Joaquin ; Klüver, Nils ; Schirmer, Kristin ; Tanneberger, Katrin ; Tobor-Kapłon, Marysia ; Witters, Hilda ; Belanger, Scott ; Benfenati, Emilio ; Creton, Stuart ; Cronin, Mark T D ; Eggen, Rik I L ; Embry, Michelle ; Ekman, Drew ; Gourmelon, Anne ; Halder, Marlies ; Hardy, Barry ; Hartung, Thomas ; Hubesch, Bruno ; Jungmann, Dirk ; Lampi, Mark A. ; Lee, Lucy ; Léonard, Marc ; Küster, Eberhard ; Lillicrap, Adam ; Luckenbach, Till ; Murk, Albertinka J. ; Navas, José M. ; Peijnenburg, Willie ; Repetto, Guillermo ; Salinas, Edward ; Schüürmann, Gerrit ; Spielmann, Horst ; Tollefsen, Knut Erik ; Walter-Rohde, Susanne ; Whale, Graham ; Wheeler, James R. ; Winter, Matthew J. / A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment. In: Regulatory Toxicology and Pharmacology. 2013 ; Vol. 67, No. 3. pp. 506-530.
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