A Drosophila third chromosome minute locus encodes a ribosomal protein

S. Andersson, S. Saeboe-Larssen, A. Lambertsson, J. Merriam, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

Minutes (M) are a group of over 50 phenotypically similar Drosophila mutations widely believed to affect ribosomal protein genes. This report describes the characterization of the P element-induced M(3)95A(Plac92) mutation [allelic to M(3)95A]. This mutation can be reversed by the mobilization of the P element, demonstrating that the mutation is caused by insertion of this transposable element. The gene interrupted by insertion of the P element was cloned by use of inverse polymerase chain reaction. Nucleotide sequence analysis revealed a 70-75% identity to the human and rat ribosomal protein S3 genes, and to the Xenopus ribosomal protein S1a gene. At the amino acid level, the overall identity is ≃ 78% for all three species. This is only the second time that a Minute has been demonstrated to encode a ribosomal protein.

Original languageEnglish (US)
Pages (from-to)513-520
Number of pages8
JournalGenetics
Volume137
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Ribosomal Proteins
Drosophila
Chromosomes
Mutation
Xenopus Proteins
Genes
DNA Transposable Elements
Insertional Mutagenesis
Sequence Analysis
Amino Acids
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Andersson, S., Saeboe-Larssen, S., Lambertsson, A., Merriam, J., & Jacobs-Lorena, M. (1994). A Drosophila third chromosome minute locus encodes a ribosomal protein. Genetics, 137(2), 513-520.

A Drosophila third chromosome minute locus encodes a ribosomal protein. / Andersson, S.; Saeboe-Larssen, S.; Lambertsson, A.; Merriam, J.; Jacobs-Lorena, Marcelo.

In: Genetics, Vol. 137, No. 2, 1994, p. 513-520.

Research output: Contribution to journalArticle

Andersson, S, Saeboe-Larssen, S, Lambertsson, A, Merriam, J & Jacobs-Lorena, M 1994, 'A Drosophila third chromosome minute locus encodes a ribosomal protein', Genetics, vol. 137, no. 2, pp. 513-520.
Andersson S, Saeboe-Larssen S, Lambertsson A, Merriam J, Jacobs-Lorena M. A Drosophila third chromosome minute locus encodes a ribosomal protein. Genetics. 1994;137(2):513-520.
Andersson, S. ; Saeboe-Larssen, S. ; Lambertsson, A. ; Merriam, J. ; Jacobs-Lorena, Marcelo. / A Drosophila third chromosome minute locus encodes a ribosomal protein. In: Genetics. 1994 ; Vol. 137, No. 2. pp. 513-520.
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