A Drosophila third chromosome minute locus encodes a ribosomal protein

S. Andersson, S. Saeboe-Larssen, A. Lambertsson, J. Merriam, M. Jacobs-Lorena

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Abstract

Minutes (M) are a group of over 50 phenotypically similar Drosophila mutations widely believed to affect ribosomal protein genes. This report describes the characterization of the P element-induced M(3)95A(Plac92) mutation [allelic to M(3)95A]. This mutation can be reversed by the mobilization of the P element, demonstrating that the mutation is caused by insertion of this transposable element. The gene interrupted by insertion of the P element was cloned by use of inverse polymerase chain reaction. Nucleotide sequence analysis revealed a 70-75% identity to the human and rat ribosomal protein S3 genes, and to the Xenopus ribosomal protein S1a gene. At the amino acid level, the overall identity is ≃ 78% for all three species. This is only the second time that a Minute has been demonstrated to encode a ribosomal protein.

Original languageEnglish (US)
Pages (from-to)513-520
Number of pages8
JournalGenetics
Volume137
Issue number2
StatePublished - Jan 1 1994
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

Cite this

Andersson, S., Saeboe-Larssen, S., Lambertsson, A., Merriam, J., & Jacobs-Lorena, M. (1994). A Drosophila third chromosome minute locus encodes a ribosomal protein. Genetics, 137(2), 513-520.