@article{c42cf014735c47ceacbb8f3505306198,
title = "A DNA integrity network in the yeast Saccharomyces cerevisiae",
abstract = "A network governing DNA integrity was identified in yeast by a global genetic analysis of synthetic fitness or lethality defect (SFL) interactions. Within this network, 16 functional modules or minipathways were defined based on patterns of global SFL interactions. Modules or genes involved in DNA replication, DNA-replication checkpoint (DRC) signaling, and oxidative stress response were identified as the major guardians against lethal spontaneous DNA damage, efficient repair of which requires the functions of the DNA-damage checkpoint signaling and multiple DNA-repair pathways. This genome-wide genetic interaction network also identified novel components (DIA2, NPT1, HST3, HST4, and the CSM1 module) that potentially contribute to mitotic DNA replication and genomic stability and revealed novel functions of well-studied genes (the CTF18 module) in DRC signaling. This network will guide more detailed characterization of mechanisms governing DNA integrity in yeast and other organisms.",
author = "Xuewen Pan and Ping Ye and Yuan, {Daniel S.} and Xiaoling Wang and Bader, {Joel S.} and Boeke, {Jef D.}",
note = "Funding Information: We thank members of the Boeke lab for valuable discussions and Pamela Meluh for critical comments on the manuscript. We thank Brian Peyser and Forrest Spencer for valuable discussions on synthetic lethality networks, Heng Zhu for the GAL1pr-GST-CTF4 and GAL1pr-GST overexpression plasmids, Alain Verreault for the GAL1pr-HHT plasmid, and Ivana Celic for sharing unpublished data. Raw data were submitted to GEO (Accession #GSE3574). We regret inability to cite many relevant studies of DNA metabolism and genomic instability due to space limits. Under a licensing agreement between Open Biosystems, Inc. and the Johns Hopkins University, the University is entitled to a share of royalties on sales of yeast strains described in this article. The terms of this arrangement are being managed by the Johns Hopkins University in accordance with its conflict of interest policies. X.P. is a fellow of the Leukemia & Lymphoma Society. J.S.B. is supported in part by the Whitaker Foundation. This work was supported in part by NHGRI grant HG02432 and by NIH Roadmap grant “Technology Center for Networks and Pathways” (RR020839) to J.D.B. P.Y. and J.S.B. prepared the Supplemental Data section titled “A Bayesian Network Approach to Identify Functional Modules.” ",
year = "2006",
month = mar,
day = "10",
doi = "10.1016/j.cell.2005.12.036",
language = "English (US)",
volume = "124",
pages = "1069--1081",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",
}