A DNA Damage-Induced, SOS-Independent Checkpoint Regulates Cell Division in Caulobacter crescentus

Joshua Modell, Tracy K. Kambara, Barrett S. Perchuk, Michael T. Laub

Research output: Contribution to journalArticle

Abstract

Cells must coordinate DNA replication with cell division, especially during episodes of DNA damage. The paradigm for cell division control following DNA damage in bacteria involves the SOS response where cleavage of the transcriptional repressor LexA induces a division inhibitor. However, in Caulobacter crescentus, cells lacking the primary SOS-regulated inhibitor, sidA, can often still delay division post-damage. Here we identify didA, a second cell division inhibitor that is induced by DNA damage, but in an SOS-independent manner. Together, DidA and SidA inhibit division, such that cells lacking both inhibitors divide prematurely following DNA damage, with lethal consequences. We show that DidA does not disrupt assembly of the division machinery and instead binds the essential division protein FtsN to block cytokinesis. Intriguingly, mutations in FtsW and FtsI, which drive the synthesis of septal cell wall material, can suppress the activity of both SidA and DidA, likely by causing the FtsW/I/N complex to hyperactively initiate cell division. Finally, we identify a transcription factor, DriD, that drives the SOS-independent transcription of didA following DNA damage.

Original languageEnglish (US)
JournalPLoS Biology
Volume12
Issue number10
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

Fingerprint

Caulobacter crescentus
Cell Division
DNA damage
DNA Damage
cell division
Sida
Cells
DNA
SOS response (genetics)
Cytokinesis
cytokinesis
DNA replication
DNA Replication
lethal genes
Cell Wall
Transcription
Transcription Factors
transcription factors
transcription (genetics)
Machinery

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

A DNA Damage-Induced, SOS-Independent Checkpoint Regulates Cell Division in Caulobacter crescentus. / Modell, Joshua; Kambara, Tracy K.; Perchuk, Barrett S.; Laub, Michael T.

In: PLoS Biology, Vol. 12, No. 10, 01.01.2014.

Research output: Contribution to journalArticle

Modell, Joshua ; Kambara, Tracy K. ; Perchuk, Barrett S. ; Laub, Michael T. / A DNA Damage-Induced, SOS-Independent Checkpoint Regulates Cell Division in Caulobacter crescentus. In: PLoS Biology. 2014 ; Vol. 12, No. 10.
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