Staphylococcus aureus suşlarinda antibiyotiǧe dirençli gen tanisi için tek kullanimlik MEMS DNA biyosensörü

Translated title of the contribution: A disposable MEMS DNA biosensor for antibiotic resistant gene detection in Staphylococcus aureus

Hatice Ceylan, Haluk Külah, Aslan Alp, Canan Özgen, Gülşen Hasçelik

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study presents combination of advantages in MEMS technology and molecular methods for detection of methicillin resistant gene, mecA, in Staphylococcus aureus. A disposable MEMS DNA biosensor was designed and fabricated for optical and electrochemical detections. Adsorption of DNA on gold electrode was examined theoretically and experimentally. In theoretical studies, Lennard Jones potential energies of gold and thiol group, and adsorption kinetics of DNA were discussed and, electrochemical analysis of Hoescht 33258 was given. In experimental studies, 27-mer capture and target probes were used to examine the detection of mecA gene. YOYO®-1-iodide nucleic acid stain was used to check adsorption on disposable gold electrodes while Hoescht 33258 was used as a redox marker to amplify signal in electrochemical detection. Experimental results obtained from fluorescence microscopy demonstrate the applicability of hybridization on gold electrodes. Detection of mecA gene electrochemically needs further study.

Translated title of the contributionA disposable MEMS DNA biosensor for antibiotic resistant gene detection in Staphylococcus aureus
Original languageTurkish
Title of host publication2010 15th National Biomedical Engineering Meeting, BIYOMUT2010
DOIs
StatePublished - Jul 15 2010
Event2010 15th National Biomedical Engineering Meeting, BIYOMUT2010 - Antalya, Turkey
Duration: Apr 21 2010Apr 24 2010

Publication series

Name2010 15th National Biomedical Engineering Meeting, BIYOMUT2010

Conference

Conference
CountryTurkey
CityAntalya
Period4/21/104/24/10

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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    Ceylan, H., Külah, H., Alp, A., Özgen, C., & Hasçelik, G. (2010). Staphylococcus aureus suşlarinda antibiyotiǧe dirençli gen tanisi için tek kullanimlik MEMS DNA biyosensörü. In 2010 15th National Biomedical Engineering Meeting, BIYOMUT2010 [5479793] (2010 15th National Biomedical Engineering Meeting, BIYOMUT2010). https://doi.org/10.1109/BIYOMUT.2010.5479793