A direct chemical interaction between dynorphin and excitatory amino acids

Amina Woods, Abraham Zangen

Research output: Contribution to journalArticle

Abstract

The endogenous opioid peptide dynorphin A elicits non-opioid receptor-mediated neurotoxic effects. These effects are blocked by pretreatment with N-methyl-D-aspartate (NMDA) receptor antagonists. Herein, the mechanism for the non-opioid effects of dynorphin and related peptides was studied by matrix-assisted laser desorption ionization (MALDI) mass-spectrometry. We observed that both glutamate or aspartate bind non-covalently to dynorphin A and dynorphin 2-17. However, when dynorphin A or dynorphin 2-17 were added to an equimolar mixture of Glutamate and Aspartate, they both complexed preferentially with glutamate. These data may explain the non-opioid physiological effects of dynorphin A and related peptides and indicate that the direct chemical interaction between neurotransmitters should be monitored when studying interactions between different neurochemical systems.

Original languageEnglish (US)
Pages (from-to)395-400
Number of pages6
JournalNeurochemical Research
Volume26
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Dynorphins
Excitatory Amino Acids
Glutamic Acid
Aspartic Acid
Peptides
Opioid Peptides
N-Methyl-D-Aspartate Receptors
Ionization
Mass spectrometry
Neurotransmitter Agents
Desorption
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Lasers
dynorphin (2-17)

Keywords

  • Aspartatic acid
  • Dynorphin
  • Glutamic acid matrix-assisted laser desorption ionization (MALDI) mass-spectrometry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

A direct chemical interaction between dynorphin and excitatory amino acids. / Woods, Amina; Zangen, Abraham.

In: Neurochemical Research, Vol. 26, No. 4, 2001, p. 395-400.

Research output: Contribution to journalArticle

Woods, Amina ; Zangen, Abraham. / A direct chemical interaction between dynorphin and excitatory amino acids. In: Neurochemical Research. 2001 ; Vol. 26, No. 4. pp. 395-400.
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