A developmental gene (Tolloid/BMP-1) is regulated in Aplysia neurons by treatments that induce long-term sensitization

Qing R. Liu, Samer Hattar, Shogo Endo, Kathleen MacPhee, Man Zhang, Leonard J. Cleary, John H. Byrne, Arnold Eskin

Research output: Contribution to journalArticle

Abstract

Long-term sensitization training, or procedures that mimic the training, produces long-term facilitation of sensory-motor neuron synapses in Aplysia. The long-term effects of these procedures require mRNA and protein synthesis (Montarolo et al., 1986; Castellucci et al., 1989). Using the techniques of differential display reverse transcription PCR (DDRT-PCR) and ribonuclease protection assays (RPA), we identified a cDNA whose mRNA level was increased significantly in sensory neurons by treatments of isolated pleural-pedal ganglia with serotonin for 1.5 hr or by long-term behavioral training of Aplysia. The effects of serotonin and behavioral training on this mRNA were mimicked by treatments that elevate cAMP. The Aplysia mRNA increased by serotonin and behavioral training was 41-45% identical to a developmentally regulated gene family which includes Drosophila tolloid and human bone morphogenetic protein-1 (BMP-1). Both tolloid and BMP-1 encode metalloproteases that might activate TGF-β (transforming growth factor β)- like molecules or process procollagens. Aplysia tolloid/BMP-1-like protein (apTBL-1) might regulate the morphology and efficacy of synaptic connections between sensory and motor neurons, which are associated with long-term sensitization.

Original languageEnglish (US)
Pages (from-to)755-764
Number of pages10
JournalJournal of Neuroscience
Volume17
Issue number2
StatePublished - 1997
Externally publishedYes

Fingerprint

Bone Morphogenetic Protein 1
Developmental Genes
Aplysia
Sensory Receptor Cells
Neurons
Serotonin
Messenger RNA
Motor Neurons
Procollagen
Transforming Growth Factors
Metalloproteases
Ribonucleases
Ganglia
Synapses
Reverse Transcription
Drosophila
Foot
Proteins
Complementary DNA
Polymerase Chain Reaction

Keywords

  • Aplysia
  • learning
  • memory
  • metalloprotease
  • sensitization
  • TGF-β
  • tolloid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Liu, Q. R., Hattar, S., Endo, S., MacPhee, K., Zhang, M., Cleary, L. J., ... Eskin, A. (1997). A developmental gene (Tolloid/BMP-1) is regulated in Aplysia neurons by treatments that induce long-term sensitization. Journal of Neuroscience, 17(2), 755-764.

A developmental gene (Tolloid/BMP-1) is regulated in Aplysia neurons by treatments that induce long-term sensitization. / Liu, Qing R.; Hattar, Samer; Endo, Shogo; MacPhee, Kathleen; Zhang, Man; Cleary, Leonard J.; Byrne, John H.; Eskin, Arnold.

In: Journal of Neuroscience, Vol. 17, No. 2, 1997, p. 755-764.

Research output: Contribution to journalArticle

Liu, QR, Hattar, S, Endo, S, MacPhee, K, Zhang, M, Cleary, LJ, Byrne, JH & Eskin, A 1997, 'A developmental gene (Tolloid/BMP-1) is regulated in Aplysia neurons by treatments that induce long-term sensitization', Journal of Neuroscience, vol. 17, no. 2, pp. 755-764.
Liu, Qing R. ; Hattar, Samer ; Endo, Shogo ; MacPhee, Kathleen ; Zhang, Man ; Cleary, Leonard J. ; Byrne, John H. ; Eskin, Arnold. / A developmental gene (Tolloid/BMP-1) is regulated in Aplysia neurons by treatments that induce long-term sensitization. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 2. pp. 755-764.
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