A dendrimer-based immunosensor for improved capture and detection of tumor necrosis factor-α cytokine

Admira Bosnjakovic, Manoj K. Mishra, Hye Jung Han, Roberto Romero, Kannan Rangaramanujam

Research output: Contribution to journalArticle

Abstract

A dendrimer-based sandwich type enzyme-linked immunosorbent assay (ELISA) was developed for the improved detection of recombinant human tumor necrosis factor-alpha (TNF-α) for early diagnosis of perinatal diseases. Hydroxyl-terminated generation four poly(amidoamine) dendrimer (G4-OH) was used for the development of a solid phase bio-sensing platform. The surface of the ELISA plate was modified with polyethylene-glycol (PEG) and thiol-functionalized G4-OH was immobilized on the PEG-functionalized plate. A capture antibody was oxidized and covalently immobilized onto the dendrimer-modified ELISA plate, which provides favorable orientation for the antigen binding sites toward the analyte. The dendrimer-modified plate showed enhanced sensitivity, and the detection limit for TNF-α was found to be 0.48pgmL -1, which is significantly better than the commercially available ELISA kit. The selectivity of the dendrimer-modified ELISA plate was further evaluated with a mixture of cytokines, which showed results for similar to that of TNF-α alone. The modified plate provides a greater opportunity for the detection of a wide range of cytokines and biomarkers.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalAnalytica Chimica Acta
Volume720
DOIs
Publication statusPublished - Mar 30 2012
Externally publishedYes

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Keywords

  • Dendrimer biosensor
  • ELISA
  • PAMAM dendrimer
  • TNF-α

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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