A cycloheximide-enhanced protein in cytomegalovirus-infected cells

Kuan Teh Jeang, D Wade Gibson

Research output: Contribution to journalArticle

Abstract

Following the treatment of cytomegalovirus (CMV, strain Colburn)-infected cells with the drug cycloheximide early after infection, dramatically enhanced amounts of an otherwise minor protein are synthesized. Size and charge estimates, based on one- and two-dimensional separations in denaturing polyacrylamide gels, indicate that this protein has a molecular weight of 94K and an acidic net charge. Results of experiments to investigate its synthesis indicate that (i) the 94K protein is expressed by 2-4 hr after infection, (ii) its appearance requires de novo RNA synthesis, (iii) both the protein and its mRNA appear to be metabolically stable, and (iv) the increased amount of this protein following cycloheximide inhibition appears to be the result of selective mRNA amplification. On the basis of its partitioning during detergent fractionation procedures, it is suggested that the 94K protein may function in the cytoplasm.

Original languageEnglish (US)
Pages (from-to)362-374
Number of pages13
JournalVirology
Volume107
Issue number2
DOIs
StatePublished - 1980

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Cycloheximide
Cytomegalovirus
Proteins
Messenger RNA
Infection
Detergents
Cytoplasm
Molecular Weight
RNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

A cycloheximide-enhanced protein in cytomegalovirus-infected cells. / Jeang, Kuan Teh; Gibson, D Wade.

In: Virology, Vol. 107, No. 2, 1980, p. 362-374.

Research output: Contribution to journalArticle

Jeang, Kuan Teh ; Gibson, D Wade. / A cycloheximide-enhanced protein in cytomegalovirus-infected cells. In: Virology. 1980 ; Vol. 107, No. 2. pp. 362-374.
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