TY - JOUR
T1 - A cycloheximide-enhanced protein in cytomegalovirus-infected cells
AU - Jeang, Kuan Teh
AU - Gibson, Wade
N1 - Funding Information:
This study was aided by Research Grant AI 13718 from the National Institute of Allergy and Infectious Diseases, and by Research Grant l-613 from the March of Dimes Birth Defects Foundation.
Funding Information:
We thank Michael Murphy for excellent technical assistance and Carol Kenyon and Barbara Spink fol help in typing the manuscript. K.T.J. was the recipient of a summer research stipend from Public Health Service Biomedical Research Support Grant RR 5378-17.
PY - 1980/12
Y1 - 1980/12
N2 - Following the treatment of cytomegalovirus (CMV, strain Colburn)-infected cells with the drug cycloheximide early after infection, dramatically enhanced amounts of an otherwise minor protein are synthesized. Size and charge estimates, based on one- and two-dimensional separations in denaturing polyacrylamide gels, indicate that this protein has a molecular weight of 94K and an acidic net charge. Results of experiments to investigate its synthesis indicate that (i) the 94K protein is expressed by 2-4 hr after infection, (ii) its appearance requires de novo RNA synthesis, (iii) both the protein and its mRNA appear to be metabolically stable, and (iv) the increased amount of this protein following cycloheximide inhibition appears to be the result of selective mRNA amplification. On the basis of its partitioning during detergent fractionation procedures, it is suggested that the 94K protein may function in the cytoplasm.
AB - Following the treatment of cytomegalovirus (CMV, strain Colburn)-infected cells with the drug cycloheximide early after infection, dramatically enhanced amounts of an otherwise minor protein are synthesized. Size and charge estimates, based on one- and two-dimensional separations in denaturing polyacrylamide gels, indicate that this protein has a molecular weight of 94K and an acidic net charge. Results of experiments to investigate its synthesis indicate that (i) the 94K protein is expressed by 2-4 hr after infection, (ii) its appearance requires de novo RNA synthesis, (iii) both the protein and its mRNA appear to be metabolically stable, and (iv) the increased amount of this protein following cycloheximide inhibition appears to be the result of selective mRNA amplification. On the basis of its partitioning during detergent fractionation procedures, it is suggested that the 94K protein may function in the cytoplasm.
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U2 - 10.1016/0042-6822(80)90304-9
DO - 10.1016/0042-6822(80)90304-9
M3 - Article
C2 - 6256937
AN - SCOPUS:0019205927
SN - 0042-6822
VL - 107
SP - 362
EP - 374
JO - Virology
JF - Virology
IS - 2
ER -