A cryptic peptide (160-169) of thyrotropin-releasing hormone prohormone demonstrates biological activity in vivo and in vitro

Frances E. Carr, Henry G. Fein, Carolyn U. Fisher, Martin W. Wessendorf, Robert C. Smallridge

Research output: Contribution to journalArticle

Abstract

TRH is synthesized as a precursor peptide containing five copies of the sequence Gln-His-Pro-Gly, QHPG, flanked by paired basic amino acids, and linked by other peptides. We tested one cryptic peptide, PPT (160-169, SFPWMESDVT), as a possible physiological regulator of pituitary activity in vivo. Male rats were cannulated (jugular) and received a single dose of either PPT or TRH (10-8-10-6 M). PPT caused no consistent effects on either TSH or PRL secretion, while TRH stimulated the secretion of both hormones. However, PPT stimulated a dose-dependent increase in both pituitary TSHβ and PRL mRNA content at 240 min similar to TRH. In primary cultures of rat pituitaries, PPT stimulated a maximum 4-fold increase in TSHβ mRNA and a 2-fold increase in PRL mRNA in 4 h, while TRH increased both TSHβ and PRL mRNA approximately 3-fold. Again, PPT had no significant effect on TSH or PRL secretion into the medium. Thus, PPT appears to be a physiological regulator of both TSH and PRL synthesis, but, unlike TRH, does not act as a secretagogue.

Original languageEnglish (US)
Pages (from-to)2653-2658
Number of pages6
JournalEndocrinology
Volume131
Issue number6
StatePublished - Dec 1992
Externally publishedYes

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Thyrotropin-Releasing Hormone
Messenger RNA
Peptides
Basic Amino Acids
Neck
Hormones
In Vitro Techniques

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Carr, F. E., Fein, H. G., Fisher, C. U., Wessendorf, M. W., & Smallridge, R. C. (1992). A cryptic peptide (160-169) of thyrotropin-releasing hormone prohormone demonstrates biological activity in vivo and in vitro. Endocrinology, 131(6), 2653-2658.

A cryptic peptide (160-169) of thyrotropin-releasing hormone prohormone demonstrates biological activity in vivo and in vitro. / Carr, Frances E.; Fein, Henry G.; Fisher, Carolyn U.; Wessendorf, Martin W.; Smallridge, Robert C.

In: Endocrinology, Vol. 131, No. 6, 12.1992, p. 2653-2658.

Research output: Contribution to journalArticle

Carr, FE, Fein, HG, Fisher, CU, Wessendorf, MW & Smallridge, RC 1992, 'A cryptic peptide (160-169) of thyrotropin-releasing hormone prohormone demonstrates biological activity in vivo and in vitro', Endocrinology, vol. 131, no. 6, pp. 2653-2658.
Carr, Frances E. ; Fein, Henry G. ; Fisher, Carolyn U. ; Wessendorf, Martin W. ; Smallridge, Robert C. / A cryptic peptide (160-169) of thyrotropin-releasing hormone prohormone demonstrates biological activity in vivo and in vitro. In: Endocrinology. 1992 ; Vol. 131, No. 6. pp. 2653-2658.
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