A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells

Peixin Huang, Sean M. Riordan, Daniel P. Heruth, Dmitry N. Grigoryev, Li Qin Zhang, Shui Qing Ye

Research output: Contribution to journalArticle

Abstract

Aging is the predominant risk factor for cardiovascular diseases and contributes to a considerably more severe outcome in patients with acute myocardial infarction. Resveratrol, a polyphenol found in red wine, is a caloric restriction mimetic with potential anti-aging properties which has emerged as a beneficial nutraceutical for patients with cardiovascular disease. Although resveratrol is widely consumed as a nutritional supplement, its mechanism of action remains to be elucidated fully. Here, we report that resveratrol activates human nicotinamide phosphoribosyltransferase (NAMPT), SIRT4 and telomerase reverse transcriptase (hTERT) in human aortic smooth muscle cells. Similar observations were obtained in resveratrol treated C57BL/6J mouse heart and liver tissues. Resverotrol can also augment telomerase activity in both human pulmonary microvascular endothelial cells and A549 cells. Blocking NAMPT and SIRT4 expression prevents induction of hTERT in human aortic smooth muscle cells while overexpression of NAMPT elevates the telomerase activity induced by resveratrol in A549 cells. Together, these results identify a NAMPT-SIRT4-hTERT axis as a novel mechanism by which resveratrol may affect the anti-aging process in human aortic smooth muscle cells, mouse hearts and other cells. These findings enrich our understanding of the positive effects of resveratrol in human cardiovascular diseases.

Original languageEnglish (US)
Pages (from-to)10812-10824
Number of pages13
JournalOncotarget
Volume6
Issue number13
StatePublished - 2015
Externally publishedYes

Fingerprint

Nicotinamide Phosphoribosyltransferase
Smooth Muscle Myocytes
Telomerase
Cardiovascular Diseases
Caloric Restriction
Polyphenols
Wine
Dietary Supplements
resveratrol
human TERT protein
Inbred C57BL Mouse
Endothelial Cells
Myocardial Infarction
Lung
Liver

Keywords

  • Aging
  • NAMPT
  • Resveratrol
  • SIRT
  • Telomerase

ASJC Scopus subject areas

  • Oncology

Cite this

Huang, P., Riordan, S. M., Heruth, D. P., Grigoryev, D. N., Zhang, L. Q., & Ye, S. Q. (2015). A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells. Oncotarget, 6(13), 10812-10824.

A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells. / Huang, Peixin; Riordan, Sean M.; Heruth, Daniel P.; Grigoryev, Dmitry N.; Zhang, Li Qin; Ye, Shui Qing.

In: Oncotarget, Vol. 6, No. 13, 2015, p. 10812-10824.

Research output: Contribution to journalArticle

Huang, P, Riordan, SM, Heruth, DP, Grigoryev, DN, Zhang, LQ & Ye, SQ 2015, 'A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells', Oncotarget, vol. 6, no. 13, pp. 10812-10824.
Huang, Peixin ; Riordan, Sean M. ; Heruth, Daniel P. ; Grigoryev, Dmitry N. ; Zhang, Li Qin ; Ye, Shui Qing. / A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells. In: Oncotarget. 2015 ; Vol. 6, No. 13. pp. 10812-10824.
@article{31cf098b5cc64f848dc8d33fd4a49b16,
title = "A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells",
abstract = "Aging is the predominant risk factor for cardiovascular diseases and contributes to a considerably more severe outcome in patients with acute myocardial infarction. Resveratrol, a polyphenol found in red wine, is a caloric restriction mimetic with potential anti-aging properties which has emerged as a beneficial nutraceutical for patients with cardiovascular disease. Although resveratrol is widely consumed as a nutritional supplement, its mechanism of action remains to be elucidated fully. Here, we report that resveratrol activates human nicotinamide phosphoribosyltransferase (NAMPT), SIRT4 and telomerase reverse transcriptase (hTERT) in human aortic smooth muscle cells. Similar observations were obtained in resveratrol treated C57BL/6J mouse heart and liver tissues. Resverotrol can also augment telomerase activity in both human pulmonary microvascular endothelial cells and A549 cells. Blocking NAMPT and SIRT4 expression prevents induction of hTERT in human aortic smooth muscle cells while overexpression of NAMPT elevates the telomerase activity induced by resveratrol in A549 cells. Together, these results identify a NAMPT-SIRT4-hTERT axis as a novel mechanism by which resveratrol may affect the anti-aging process in human aortic smooth muscle cells, mouse hearts and other cells. These findings enrich our understanding of the positive effects of resveratrol in human cardiovascular diseases.",
keywords = "Aging, NAMPT, Resveratrol, SIRT, Telomerase",
author = "Peixin Huang and Riordan, {Sean M.} and Heruth, {Daniel P.} and Grigoryev, {Dmitry N.} and Zhang, {Li Qin} and Ye, {Shui Qing}",
year = "2015",
language = "English (US)",
volume = "6",
pages = "10812--10824",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals",
number = "13",

}

TY - JOUR

T1 - A critical role of nicotinamide phosphoribosyltransferase in human telomerase reverse transcriptase induction by resveratrol in aortic smooth muscle cells

AU - Huang, Peixin

AU - Riordan, Sean M.

AU - Heruth, Daniel P.

AU - Grigoryev, Dmitry N.

AU - Zhang, Li Qin

AU - Ye, Shui Qing

PY - 2015

Y1 - 2015

N2 - Aging is the predominant risk factor for cardiovascular diseases and contributes to a considerably more severe outcome in patients with acute myocardial infarction. Resveratrol, a polyphenol found in red wine, is a caloric restriction mimetic with potential anti-aging properties which has emerged as a beneficial nutraceutical for patients with cardiovascular disease. Although resveratrol is widely consumed as a nutritional supplement, its mechanism of action remains to be elucidated fully. Here, we report that resveratrol activates human nicotinamide phosphoribosyltransferase (NAMPT), SIRT4 and telomerase reverse transcriptase (hTERT) in human aortic smooth muscle cells. Similar observations were obtained in resveratrol treated C57BL/6J mouse heart and liver tissues. Resverotrol can also augment telomerase activity in both human pulmonary microvascular endothelial cells and A549 cells. Blocking NAMPT and SIRT4 expression prevents induction of hTERT in human aortic smooth muscle cells while overexpression of NAMPT elevates the telomerase activity induced by resveratrol in A549 cells. Together, these results identify a NAMPT-SIRT4-hTERT axis as a novel mechanism by which resveratrol may affect the anti-aging process in human aortic smooth muscle cells, mouse hearts and other cells. These findings enrich our understanding of the positive effects of resveratrol in human cardiovascular diseases.

AB - Aging is the predominant risk factor for cardiovascular diseases and contributes to a considerably more severe outcome in patients with acute myocardial infarction. Resveratrol, a polyphenol found in red wine, is a caloric restriction mimetic with potential anti-aging properties which has emerged as a beneficial nutraceutical for patients with cardiovascular disease. Although resveratrol is widely consumed as a nutritional supplement, its mechanism of action remains to be elucidated fully. Here, we report that resveratrol activates human nicotinamide phosphoribosyltransferase (NAMPT), SIRT4 and telomerase reverse transcriptase (hTERT) in human aortic smooth muscle cells. Similar observations were obtained in resveratrol treated C57BL/6J mouse heart and liver tissues. Resverotrol can also augment telomerase activity in both human pulmonary microvascular endothelial cells and A549 cells. Blocking NAMPT and SIRT4 expression prevents induction of hTERT in human aortic smooth muscle cells while overexpression of NAMPT elevates the telomerase activity induced by resveratrol in A549 cells. Together, these results identify a NAMPT-SIRT4-hTERT axis as a novel mechanism by which resveratrol may affect the anti-aging process in human aortic smooth muscle cells, mouse hearts and other cells. These findings enrich our understanding of the positive effects of resveratrol in human cardiovascular diseases.

KW - Aging

KW - NAMPT

KW - Resveratrol

KW - SIRT

KW - Telomerase

UR - http://www.scopus.com/inward/record.url?scp=84929648706&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929648706&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 10812

EP - 10824

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 13

ER -