A conserved proline triplet in Val-tRNA synthetase and the origin of elongation factor P

Agata L. Starosta, Jürgen Lassak, Lauri Peil, Gemma C. Atkinson, Christopher Woolstenhulme, Kai Virumäe, Allen Buskirk, Tanel Tenson, Jaanus Remme, Kirsten Jung, Daniel N. Wilson

Research output: Contribution to journalArticle

Abstract

Bacterial ribosomes stall on polyproline stretches and require the elongation factor P (EF-P) to relieve the arrest. Yet it remains unclear why evolution has favored the development of EF-P rather than selecting against the occurrence of polyproline stretches in proteins. We have discovered that only a single polyproline stretch is invariant across all domains of life, namely a proline triplet in ValS, the tRNA synthetase, that charges tRNAVal with valine. Here, we show that expression of ValS in vivo and in vitro requires EF-P and demonstrate that the proline triplet located in the active site of ValS is important for efficient charging of tRNAVal with valine and preventing formation of mischarged Thr-tRNAVal as well as efficient growth of E. coli in vivo. We suggest that the critical role of the proline triplet for ValS activity may explain why bacterial cells coevolved the EF-P rescue system.

Original languageEnglish (US)
Pages (from-to)476-483
Number of pages8
JournalCell Reports
Volume9
Issue number2
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Valsartan
Amino Acyl-tRNA Synthetases
RNA, Transfer, Val
Proline
Valine
Ribosomes
Escherichia coli
Catalytic Domain
factor EF-P
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Starosta, A. L., Lassak, J., Peil, L., Atkinson, G. C., Woolstenhulme, C., Virumäe, K., ... Wilson, D. N. (2014). A conserved proline triplet in Val-tRNA synthetase and the origin of elongation factor P. Cell Reports, 9(2), 476-483. https://doi.org/10.1016/j.celrep.2014.09.008

A conserved proline triplet in Val-tRNA synthetase and the origin of elongation factor P. / Starosta, Agata L.; Lassak, Jürgen; Peil, Lauri; Atkinson, Gemma C.; Woolstenhulme, Christopher; Virumäe, Kai; Buskirk, Allen; Tenson, Tanel; Remme, Jaanus; Jung, Kirsten; Wilson, Daniel N.

In: Cell Reports, Vol. 9, No. 2, 2014, p. 476-483.

Research output: Contribution to journalArticle

Starosta, AL, Lassak, J, Peil, L, Atkinson, GC, Woolstenhulme, C, Virumäe, K, Buskirk, A, Tenson, T, Remme, J, Jung, K & Wilson, DN 2014, 'A conserved proline triplet in Val-tRNA synthetase and the origin of elongation factor P', Cell Reports, vol. 9, no. 2, pp. 476-483. https://doi.org/10.1016/j.celrep.2014.09.008
Starosta, Agata L. ; Lassak, Jürgen ; Peil, Lauri ; Atkinson, Gemma C. ; Woolstenhulme, Christopher ; Virumäe, Kai ; Buskirk, Allen ; Tenson, Tanel ; Remme, Jaanus ; Jung, Kirsten ; Wilson, Daniel N. / A conserved proline triplet in Val-tRNA synthetase and the origin of elongation factor P. In: Cell Reports. 2014 ; Vol. 9, No. 2. pp. 476-483.
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AU - Buskirk, Allen

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