A comparison of the structural polypeptides of five strains of mumps virus

M. McCarthy, Richard Johnson

Research output: Contribution to journalArticle

Abstract

The polypeptide patterns of five strains of mumps virus have been compared under uniform electrophoresis conditions. These strains included the MJ and RW strains, recently isolated from human cerebrospinal fluid, the neuro-adapted Kilham strain and the chick embryo-adapted Enders and Jeryl Lynn strains. A similar pattern of polypeptides, featuring six common and virus-specific species, was obtained with purified virus grown in ovo, in chick embryo fibroblasts (CEF) or in Vero cells. These polypeptides have been designated VP1 (mol. wt. 200 000), VP2 (mol. wt. 80 000 or 75 000), VP3 (mol. wt. 68 000), VP4 (mol. wt. 58 000), VP5 (mol. wt. 45 000), VP6 (mol. wt. 39 000). The virus glycoproteins were identified as VP2 and VP4; the nucleocapsid-associated proteins as VP1, VP3 and VP5. Additional polypeptides occurring both in uninfected Vero cells and in uninfected CEFs appeared in polypeptide profiles of purified mumps virus preparations, and could not be designated virus-specific polypeptides. Although strain differences were minimal, we did observe an extra protein, nucleocapsid-associated, in patterns from the chick-adapted Enders strain of mumps virus and strain-dependent variability in the size of VP2.

Original languageEnglish (US)
Pages (from-to)15-27
Number of pages13
JournalJournal of General Virology
Volume46
Issue number1
StatePublished - 1980

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Mumps virus
Peptides
Viruses
Nucleocapsid Proteins
Vero Cells
Chick Embryo
Cerebrospinal Fluid
Electrophoresis
Glycoproteins
Fibroblasts

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

A comparison of the structural polypeptides of five strains of mumps virus. / McCarthy, M.; Johnson, Richard.

In: Journal of General Virology, Vol. 46, No. 1, 1980, p. 15-27.

Research output: Contribution to journalArticle

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