A comparison of non-integrating reprogramming methods

Thorsten M. Schlaeger, Laurence Daheron, Thomas R. Brickler, Samuel Entwisle, Karrie Chan, Amelia Cianci, Alexander DeVine, Andrew Ettenger, Kelly Fitzgerald, Michelle Godfrey, Dipti Gupta, Jade McPherson, Prerana Malwadkar, Manav Gupta, Blair Bell, Akiko Doi, Namyoung Jung, Xin Li, Maureen S. Lynes, Emily BrookesAnne B.C. Cherry, Didem Demirbas, Alexander M. Tsankov, Leonard I. Zon, Lee L. Rubin, Andrew P. Feinberg, Alexander Meissner, Chad A. Cowan, George Q. Daley

Research output: Contribution to journalArticlepeer-review

Abstract

Human induced pluripotent stem cells (hiPSCs)1-3 are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV)4, episomal (Epi)5 and mRNA transfection mRNA methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.

Original languageEnglish (US)
Pages (from-to)58-63
Number of pages6
JournalNature biotechnology
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2015

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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