TY - JOUR
T1 - A Comparison of gel (Hydragel 30) and capillary (Capillarys III Tera) electrophoresis for the characterization of human serum proteins
AU - Howard, Brittney M.
AU - Kuh, Annie
AU - Rezavi, Lu Ann
AU - Caturegli, Patrizio
N1 - Publisher Copyright:
© 2021 The Author(s)
PY - 2021/5
Y1 - 2021/5
N2 - Objectives: To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins. Design and methods: 304 sera tested by gel electrophoresis during 8 routine laboratory days were concurrently tested by capillary electrophoresis. Gels were read by an experienced medical technologist while capillary profiles by a Sebia representative and the same technologist. Most sera (214 of 304, 70%) were also analyzed by immunofixation electrophoresis, used here as the gold standard to calculate sensitivity and specificity of the gel and capillary systems. Results: Gel and capillary estimated the concentration of albumin, gamma region, and M-spikes nearly perfectly, and that of beta, alpha-2, and alpha-1 regions with excellent correlation. The two systems classified concordantly 268 of 304 sera (88% agreement) as having no, one, or two M-spikes, but differed in the remaining 36 sera (12%). Gel electrophoresis correctly identified M-spikes in 82 of 112 sera that were shown to have monoclonal band(s) by immunofixation (73% sensitivity), and correctly did not reveal M-spikes in 97 of the 102 sera that had no immunofixation bands (95% specificity). Capillary achieved slightly higher sensitivity (85 of 112, 76%) and slightly lower specificity (94 of 102, 92%), but the two areas under the ROC curves were nearly identical at 0.84. Conclusions: Gel and capillary electrophoresis systems perform similarly to estimate the concentration of serum protein fractions and detect M-spikes.
AB - Objectives: To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins. Design and methods: 304 sera tested by gel electrophoresis during 8 routine laboratory days were concurrently tested by capillary electrophoresis. Gels were read by an experienced medical technologist while capillary profiles by a Sebia representative and the same technologist. Most sera (214 of 304, 70%) were also analyzed by immunofixation electrophoresis, used here as the gold standard to calculate sensitivity and specificity of the gel and capillary systems. Results: Gel and capillary estimated the concentration of albumin, gamma region, and M-spikes nearly perfectly, and that of beta, alpha-2, and alpha-1 regions with excellent correlation. The two systems classified concordantly 268 of 304 sera (88% agreement) as having no, one, or two M-spikes, but differed in the remaining 36 sera (12%). Gel electrophoresis correctly identified M-spikes in 82 of 112 sera that were shown to have monoclonal band(s) by immunofixation (73% sensitivity), and correctly did not reveal M-spikes in 97 of the 102 sera that had no immunofixation bands (95% specificity). Capillary achieved slightly higher sensitivity (85 of 112, 76%) and slightly lower specificity (94 of 102, 92%), but the two areas under the ROC curves were nearly identical at 0.84. Conclusions: Gel and capillary electrophoresis systems perform similarly to estimate the concentration of serum protein fractions and detect M-spikes.
KW - Capillary
KW - Capillarys III tera
KW - Electrophoresis
KW - Hydrasys II
KW - Sebia
KW - Serum proteins
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U2 - 10.1016/j.plabm.2021.e00233
DO - 10.1016/j.plabm.2021.e00233
M3 - Article
C2 - 34095418
AN - SCOPUS:85105346725
SN - 2352-5517
VL - 25
JO - Practical Laboratory Medicine
JF - Practical Laboratory Medicine
M1 - e00233
ER -