A recently introduced system for antibody detection (REACT(TM)) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT(TM) system to a conventional tube low-ionic- strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method.
|Original language||English (US)|
|Number of pages||3|
|State||Published - Jun 7 1999|
- Affinity column
- Antibody detection
ASJC Scopus subject areas
- Immunology and Allergy