A comparison of a new affinity column system with a conventional tube LISS-antiglobulin test for antibody detection

R. S. Shirey, J. S. Boyd, C. Barrasso, K. E. Kingand, P. M. Ness

Research output: Contribution to journalArticlepeer-review

Abstract

A recently introduced system for antibody detection (REACT(TM)) consists of affinity columns (AFC) that contain protein A and protein G-coated agarose. We compared the ReACT(TM) system to a conventional tube low-ionic- strength saline antiglobulin test (LISS-AGT). We selected 100 LISS-AGT positive samples with clinically important and benign antibodies of varying strengths and 130 LISS-AGT negative samples to evaluate by the AFC method. AFC tests were positive with all 84 clinically important antibodies, including 36 antibodies that reacted ≤ 1+ at LISS-AGT (0% falsely negative). Eleven of 16 (69%) clinically benign antibodies reacted by AFC. Five samples (2 anti-Sda, 2 anti-I, and 1 inconclusive) were negative by AFC. AFC tests were negative with all 130 samples that were negative by LISS-AGT (0% falsely positive). The AFC method showed results comparable with results obtained with a conventional tube LISS-AGT for detection of clinically important antibodies. Some unwanted, clinically benign antibodies may not be detected by the AFC method.

Original languageEnglish (US)
Pages (from-to)75-77
Number of pages3
JournalImmunohematology
Volume15
Issue number2
StatePublished - Jun 7 1999

Keywords

  • Affinity column
  • Antibody detection
  • Microcolumn

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology

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