TY - JOUR
T1 - A comparison between microsatellite and quantitative PCR analyses to detect frequent p16 copy number changes in primary bladder tumors
AU - Cairns, John Paul
AU - Chiang, Pei Wen
AU - Ramamoorthy, Saravanan
AU - Kurnit, David M.
AU - Sidransky, David
PY - 1998/2
Y1 - 1998/2
N2 - We tested 70 primary bladder tumors for altered copy number of p16 (D9S1752) by microsatellite analysis and by a quantitative PCR (QPCR) assay. These two approaches were fully concordant for 53 tumors, including all 39 tumors in which microsatellite analysis detected loss. In addition, the QPCR method detected useful anomalies in 17 additional cases, including those in which D9S1752 was uninformative. QPCR was abnormal in 56 of 70 (80%) cases, whereas microsatellite analysis was abnormal in 39 of 70 (56%) cases. Although QPCR uses more DNA than microsatellite analysis, it represents a rapid, informative technique that can readily detect both chromosome 9p21 deletions and amplifications in primary bladder tumors without the need for electrophoretic separation.
AB - We tested 70 primary bladder tumors for altered copy number of p16 (D9S1752) by microsatellite analysis and by a quantitative PCR (QPCR) assay. These two approaches were fully concordant for 53 tumors, including all 39 tumors in which microsatellite analysis detected loss. In addition, the QPCR method detected useful anomalies in 17 additional cases, including those in which D9S1752 was uninformative. QPCR was abnormal in 56 of 70 (80%) cases, whereas microsatellite analysis was abnormal in 39 of 70 (56%) cases. Although QPCR uses more DNA than microsatellite analysis, it represents a rapid, informative technique that can readily detect both chromosome 9p21 deletions and amplifications in primary bladder tumors without the need for electrophoretic separation.
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M3 - Article
C2 - 9516934
AN - SCOPUS:0031913996
SN - 1078-0432
VL - 4
SP - 441
EP - 444
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 2
ER -