A comparative field study of radiolabeled and enzyme-conjugated synthetic DNA probes for the diagnosis of falciparum malaria

O. Sethabutr, A. E. Brown, J. Gingrich, H. K. Webster, N. Pooyindee, D. N. Taylor, P. Echeverria

Research output: Contribution to journalArticle

Abstract

Synthetic, 21-base, DNA probes to the genome of Plasmodium falciparum were either 32P-labeled or enzyme-conjugated for comparative field studies. The sensitivity of both probes was compared with microscopy in the examination of blood samples from 97 Thai villagers, 47 Thai Rangers, and 19 malaria-free Bangkok residents. The probes were also used to monitor the therapeutic response of 18 of the Rangers during 7 days of treatment. The probes proved highly specific. Both probes had lower limits of detection of about 100 parasites per μl blood. Thus, the low parasite densities in partially immune villagers from an endemic area were often missed, while higher parasite densities in the nonimmune Rangers were usually detected. As monitors of response to treatment, the probes paralleled microscopy in identifying reversion from positive to negative parasitemia. The enzymelabeled DNA probe as shown to perform similarly to the radiolabeled probe in populations with different malarial immune status and during curative treatment.

Original languageEnglish (US)
Pages (from-to)227-231
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume39
Issue number3
StatePublished - 1988
Externally publishedYes

Fingerprint

Falciparum Malaria
DNA Probes
Parasites
Microscopy
Enzymes
Parasitemia
Plasmodium falciparum
Malaria
Limit of Detection
Genome
Population
Therapeutics

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Sethabutr, O., Brown, A. E., Gingrich, J., Webster, H. K., Pooyindee, N., Taylor, D. N., & Echeverria, P. (1988). A comparative field study of radiolabeled and enzyme-conjugated synthetic DNA probes for the diagnosis of falciparum malaria. American Journal of Tropical Medicine and Hygiene, 39(3), 227-231.

A comparative field study of radiolabeled and enzyme-conjugated synthetic DNA probes for the diagnosis of falciparum malaria. / Sethabutr, O.; Brown, A. E.; Gingrich, J.; Webster, H. K.; Pooyindee, N.; Taylor, D. N.; Echeverria, P.

In: American Journal of Tropical Medicine and Hygiene, Vol. 39, No. 3, 1988, p. 227-231.

Research output: Contribution to journalArticle

Sethabutr, O, Brown, AE, Gingrich, J, Webster, HK, Pooyindee, N, Taylor, DN & Echeverria, P 1988, 'A comparative field study of radiolabeled and enzyme-conjugated synthetic DNA probes for the diagnosis of falciparum malaria', American Journal of Tropical Medicine and Hygiene, vol. 39, no. 3, pp. 227-231.
Sethabutr, O. ; Brown, A. E. ; Gingrich, J. ; Webster, H. K. ; Pooyindee, N. ; Taylor, D. N. ; Echeverria, P. / A comparative field study of radiolabeled and enzyme-conjugated synthetic DNA probes for the diagnosis of falciparum malaria. In: American Journal of Tropical Medicine and Hygiene. 1988 ; Vol. 39, No. 3. pp. 227-231.
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