TY - JOUR
T1 - A compact time‐of‐flight mass spectrometer for the structural analysis of biological molecules using laser desorption
AU - Cornish, Timothy
AU - Cotter, Robert J.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1992/4
Y1 - 1992/4
N2 - A compact laser‐desorption time‐of‐flight mass spectrometer using a 600 ps nitrogen laser and 1 Gsample/s transient recorder is described. The instrument incorporates two electrically‐isolatable, reflecting flight tubes designed for subsequent configuration of the mass spectrometer as a tandem instrument. In this first report, we compare mass resolution in the laser‐desorption mass spectra of an organic dye, sinapinic and caffeic acid matrices, and sevesral small peptides. For directly desorbed ions, peak widths are generally of the order of 11 to 13 ns, so that mass sesolutiojn increases with increasing mass. For peptide ions in the range of 500 to 1000 u, formed by matrix‐assisted desorption, peak widths range from 9.5 to 17.6 ns and increase with mass. However, better than unit mass resolution (1600 to 2400) is maintained throughout this range. Mass measurement accuracy is better than 0.1 u using a single calibration peak and a prompt start trigger pulse. Prospects for extending mass range and resolution are discussed.
AB - A compact laser‐desorption time‐of‐flight mass spectrometer using a 600 ps nitrogen laser and 1 Gsample/s transient recorder is described. The instrument incorporates two electrically‐isolatable, reflecting flight tubes designed for subsequent configuration of the mass spectrometer as a tandem instrument. In this first report, we compare mass resolution in the laser‐desorption mass spectra of an organic dye, sinapinic and caffeic acid matrices, and sevesral small peptides. For directly desorbed ions, peak widths are generally of the order of 11 to 13 ns, so that mass sesolutiojn increases with increasing mass. For peptide ions in the range of 500 to 1000 u, formed by matrix‐assisted desorption, peak widths range from 9.5 to 17.6 ns and increase with mass. However, better than unit mass resolution (1600 to 2400) is maintained throughout this range. Mass measurement accuracy is better than 0.1 u using a single calibration peak and a prompt start trigger pulse. Prospects for extending mass range and resolution are discussed.
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U2 - 10.1002/rcm.1290060404
DO - 10.1002/rcm.1290060404
M3 - Article
C2 - 1571597
AN - SCOPUS:0026843474
SN - 0951-4198
VL - 6
SP - 242
EP - 248
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 4
ER -