A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS

Zhuhao Wu, Lora B. Sweeney, Joseph C. Ayoob, Kayam Chak, Benjamin J. Andreone, Tomoko Ohyama, Rex Kerr, Liqun Luo, Marta Zlatic, Alex L. Kolodkin

Research output: Contribution to journalArticle

Abstract

Longitudinal axon fascicles within the Drosophila embryonic CNS provide connections between body segments and are required for coordinated neural signaling along the anterior-posterior axis. We show here that establishment of select CNS longitudinal tracts and formation of precise mechanosensory afferent innervation to the same CNS region are coordinately regulated by the secreted semaphorins Sema-2a and Sema-2b. Both Sema-2a and Sema-2b utilize the same neuronal receptor, plexin B (PlexB), but serve distinct guidance functions. Localized Sema-2b attraction promotes the initial assembly of a subset of CNS longitudinal projections and subsequent targeting of chordotonal sensory afferent axons to these same longitudinal connectives, whereas broader Sema-2a repulsion serves to prevent aberrant innervation. In the absence of Sema-2b or PlexB, chordotonal afferent connectivity within the CNS is severely disrupted, resulting in specific larval behavioral deficits. These results reveal that distinct semaphorin-mediated guidance functions converge at PlexB and are critical for functional neural circuit assembly.

Original languageEnglish (US)
Pages (from-to)281-298
Number of pages18
JournalNeuron
Volume70
Issue number2
DOIs
StatePublished - Apr 28 2011

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS'. Together they form a unique fingerprint.

  • Cite this

    Wu, Z., Sweeney, L. B., Ayoob, J. C., Chak, K., Andreone, B. J., Ohyama, T., Kerr, R., Luo, L., Zlatic, M., & Kolodkin, A. L. (2011). A Combinatorial Semaphorin Code Instructs the Initial Steps of Sensory Circuit Assembly in the Drosophila CNS. Neuron, 70(2), 281-298. https://doi.org/10.1016/j.neuron.2011.02.050