A combinatorial approach to the selective capture of circulating malignant epithelial cells by peptide ligands

Saurabh Aggarwal, Samuel Janssen, Randy M. Wadkins, James L. Harden, Samuel R. Denmeade

Research output: Contribution to journalArticle

Abstract

Early detection is critical in the administration of definitive and curative therapy of cancer. However, current detection methods are ineffective at identifying the presence of circulating metastatic cancer cells in the blood because they typically sample only a relatively small volume of blood. One strategy for sampling larger blood volumes would be to capture circulating cells in vivo over an extended period of time. The development of such a method would be substantially facilitated by the identification of peptide ligands that bind selectively to metastatic cancer cells in the blood with high affinity. To identify such ligands a combinatorial peptide library was synthesized on polyethylene acrylamide (PEGA) resin and screened for binding to malignant epithelial cells. Using Biacore, cell binding assays were performed to demonstrate that peptides selected from PEGA bead screen can bind selectively to malignant epithelial cancer cells and not to circulating leukocytes under physiologic shear stress conditions. One peptide, with the sequence QMARIPKRLARH, was used to demonstrate selective labeling of malignant epithelial cells spiked in whole blood. When immobilized on appropriate surfaces, these peptides could be used in both in vivo and ex vivo cell separation devices to efficiently and selectively capture metastatic epithelial cancer cells from flowing blood.

Original languageEnglish (US)
Pages (from-to)6077-6086
Number of pages10
JournalBiomaterials
Volume26
Issue number30
DOIs
StatePublished - Oct 1 2005

Keywords

  • Biosensor
  • Catheter
  • Cell adhesion
  • Epithelial cell
  • Peptide

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Fingerprint Dive into the research topics of 'A combinatorial approach to the selective capture of circulating malignant epithelial cells by peptide ligands'. Together they form a unique fingerprint.

  • Cite this