A coiled-coil-repeat protein 'Ccrp' in Bdellovibrio bacteriovorus prevents cellular indentation, but is not essential for vibroid cell morphology

Andrew K. Fenton, Laura Hobley, Carmen Butan, Sriram Subramaniam, Renee E. Sockett

Research output: Contribution to journalArticle

Abstract

Bdellovibrio bacteriovorus are small, vibroid, predatory bacteria that grow within the periplasmic space of a host Gram-negative bacterium. The intermediate-filament (IF)-like protein crescentin is a member of a broad class of IF-like, coiled-coil-repeat-proteins (CCRPs), discovered in Caulobacter crescentus, where it contributes to the vibroid cell shape. The B. bacteriovorus genome has a single ccrp gene encoding a protein with an unusually long, stutter-free, coiled-coil prediction; the inactivation of this did not alter the vibriod cell shape, but caused cell deformations, visualized as chiselled insets or dents, near the cell poles and a general 'creased' appearance, under the negative staining preparation used for electron microscopy, but not in unstained, frozen, hydrated cells. Bdellovibrio bacteriovorus expressing 'teal' fluorescent protein (mTFP), as a C-terminal tag on the wild-type Ccrp protein, did not deform under negative staining, suggesting that the function was not impaired. Localization of fluorescent Ccrp-mTFP showed some bias to the cell poles, independent of the cytoskeleton, as demonstrated by the addition of the MreB-specific inhibitor A22. We suggest that the Ccrp protein in B. bacteriovorus contributes as an underlying scaffold, similar to that described for the CCRP protein FilP in Streptomyces coelicolor, preventing cellular indentation, but not contributing to the vibroid shape of the B. bacteriovorus cells.

Original languageEnglish (US)
Pages (from-to)89-95
Number of pages7
JournalFEMS Microbiology Letters
Volume313
Issue number2
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Negative Staining
Proteins
Cell Shape
Caulobacter crescentus
Streptomyces coelicolor
Intermediate Filament Proteins
Periplasm
Intermediate Filaments
Gram-Negative Bacteria
Cytoskeleton
Bdellovibrio bacteriovorus
Electron Microscopy
Genome
Bacteria
Genes
IgA receptor

Keywords

  • Bdellovibrio bacteriovorus
  • Ccrp
  • Cytoskeleton
  • Intermediate filament

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

A coiled-coil-repeat protein 'Ccrp' in Bdellovibrio bacteriovorus prevents cellular indentation, but is not essential for vibroid cell morphology. / Fenton, Andrew K.; Hobley, Laura; Butan, Carmen; Subramaniam, Sriram; Sockett, Renee E.

In: FEMS Microbiology Letters, Vol. 313, No. 2, 12.2010, p. 89-95.

Research output: Contribution to journalArticle

Fenton, Andrew K. ; Hobley, Laura ; Butan, Carmen ; Subramaniam, Sriram ; Sockett, Renee E. / A coiled-coil-repeat protein 'Ccrp' in Bdellovibrio bacteriovorus prevents cellular indentation, but is not essential for vibroid cell morphology. In: FEMS Microbiology Letters. 2010 ; Vol. 313, No. 2. pp. 89-95.
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