A cluster of three novel Ca2+ channel γ subunit genes on chromosome 19q13.4: Evolution and expression profile of the γ subunit gene family

Daniel L. Burgess, Lisa A. Gefrides, Perry J. Foreman, Jeffrey L. Noebels

Research output: Contribution to journalArticlepeer-review

Abstract

The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory γ subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of γ subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel γ subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the γ subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed thai CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of γ subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.

Original languageEnglish (US)
Pages (from-to)339-350
Number of pages12
JournalGenomics
Volume71
Issue number3
DOIs
StatePublished - Feb 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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