TY - JOUR
T1 - A cluster of three novel Ca2+ channel γ subunit genes on chromosome 19q13.4
T2 - Evolution and expression profile of the γ subunit gene family
AU - Burgess, Daniel L.
AU - Gefrides, Lisa A.
AU - Foreman, Perry J.
AU - Noebels, Jeffrey L.
N1 - Funding Information:
This work was supported by NIH NS29709 (J.L.N.), by the Blue Bird Foundation for Pediatric Neurology (J.L.N., D.L.B.), and by the National EpiFellows Foundation and the Kempner Foundation (P.J.F.).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001/2/1
Y1 - 2001/2/1
N2 - The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory γ subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of γ subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel γ subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the γ subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed thai CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of γ subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.
AB - The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory γ subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of γ subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel γ subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the γ subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed thai CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of γ subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.
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U2 - 10.1006/geno.2000.6440
DO - 10.1006/geno.2000.6440
M3 - Article
C2 - 11170751
AN - SCOPUS:0035241969
SN - 0888-7543
VL - 71
SP - 339
EP - 350
JO - Genomics
JF - Genomics
IS - 3
ER -