A cluster of three novel Ca2+ channel γ subunit genes on chromosome 19q13.4: Evolution and expression profile of the γ subunit gene family

Daniel L. Burgess, Lisa A. Gefrides, Perry Foreman, Jeffrey L. Noebels

Research output: Contribution to journalArticle

Abstract

The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory γ subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of γ subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel γ subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the γ subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed thai CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of γ subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.

Original languageEnglish (US)
Pages (from-to)339-350
Number of pages12
JournalGenomics
Volume71
Issue number3
DOIs
StatePublished - Feb 1 2001
Externally publishedYes

Fingerprint

Transcriptome
Chromosomes
Genes
Brain
Protein Isoforms
Membrane Proteins
Skeletal Muscle
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

A cluster of three novel Ca2+ channel γ subunit genes on chromosome 19q13.4 : Evolution and expression profile of the γ subunit gene family. / Burgess, Daniel L.; Gefrides, Lisa A.; Foreman, Perry; Noebels, Jeffrey L.

In: Genomics, Vol. 71, No. 3, 01.02.2001, p. 339-350.

Research output: Contribution to journalArticle

@article{0162790f26ed47549e319db8a09afc9a,
title = "A cluster of three novel Ca2+ channel γ subunit genes on chromosome 19q13.4: Evolution and expression profile of the γ subunit gene family",
abstract = "The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory γ subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of γ subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel γ subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the γ subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed thai CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of γ subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.",
author = "Burgess, {Daniel L.} and Gefrides, {Lisa A.} and Perry Foreman and Noebels, {Jeffrey L.}",
year = "2001",
month = "2",
day = "1",
doi = "10.1006/geno.2000.6440",
language = "English (US)",
volume = "71",
pages = "339--350",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - A cluster of three novel Ca2+ channel γ subunit genes on chromosome 19q13.4

T2 - Evolution and expression profile of the γ subunit gene family

AU - Burgess, Daniel L.

AU - Gefrides, Lisa A.

AU - Foreman, Perry

AU - Noebels, Jeffrey L.

PY - 2001/2/1

Y1 - 2001/2/1

N2 - The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory γ subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of γ subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel γ subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the γ subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed thai CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of γ subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.

AB - The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory γ subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of γ subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel γ subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the γ subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed thai CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of γ subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.

UR - http://www.scopus.com/inward/record.url?scp=0035241969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035241969&partnerID=8YFLogxK

U2 - 10.1006/geno.2000.6440

DO - 10.1006/geno.2000.6440

M3 - Article

C2 - 11170751

AN - SCOPUS:0035241969

VL - 71

SP - 339

EP - 350

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -