A clinical evaluation of high fluorescent platelet fraction percentage in thrombocytopenia

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We evaluated an automated method (XE-2100 automated blood cell counter equipped with a laser and upgraded software, Sysmex, Kobe, Japan) to quantify the high fluorescent platelet fraction percentage (HFPF%) as an indicator of platelet production. The mean HFPF% value was 3.1 in 80 healthy subjects (95% confidence interval, 2.8%-3.5%). The coefficient of variation was less than 10% for elevated values and 10% to 20% for normal values. Sample stability was up to 48 hours. We tested the HFPF% in 171 patients with thrombocytopenia. The highest values were found in patients with autoimmune thrombocytopenia and disseminated intravascular coagulation, although HFPF% also was increased in patients with regenerating marrows. In other patient groups with decreased platelet production, the HFPF% was within the normal range. The HFPF% was predictive in the evaluation of thrombocytopenia. We found elevated HFPF% values in disorders associated with increased platelet production, particularly associated with platelet destruction, and normal values in disorders associated with decreased platelet production. The results are precise: testing can be performed as readily as a CBC count using an EDTA-anticoagulated blood sample.

Original languageEnglish (US)
Pages (from-to)282-287
Number of pages6
JournalAmerican journal of clinical pathology
Issue number2
StatePublished - Feb 2006


  • Cell analyzers
  • Platelets
  • Thrombocytopenia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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