A change in the structure of Vβ chromatin associated with TCR β allelic exclusion

Rajkamal Tripathi, Annette Jackson, Michael S. Krangel

Research output: Contribution to journalArticle

Abstract

To investigate chromatin control of TCR β rearrangement and allelic exclusion, we analyzed TCR β chromatin structure in double negative (DN) thymocytes, which are permissive for TCR β recombination, and in double positive (DP) thymocytes, which are postallelic exclusion and nonpermissive for Vβ to DβJβ recombination. Histone acetylation mapping and DNase I sensitivity studies indicate Vβ and DβJβ segments to be hyperacetylated and accessible in DN thymocytes. However, they are separated from each other by hypoacetylated and inaccessible trypsinogen chromatin. The transition from DN to DP is accompanied by selective down-regulation of Vβ acetylation and accessibility. The level of DP acetylation and accessibility is minimal for five of six Vβ segments studied but remains substantial for one. Hence, the observed changes in Vβ chromatin structure appear sufficient to account for allelic exclusion of many Vβ segments. They may contribute to, but not by themselves fully account for, allelic exclusion of others.

Original languageEnglish (US)
Pages (from-to)2316-2324
Number of pages9
JournalJournal of Immunology
Volume168
Issue number5
StatePublished - Mar 1 2002
Externally publishedYes

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Chromatin
Thymocytes
Acetylation
Genetic Recombination
Trypsinogen
Deoxyribonuclease I
Histones
Down-Regulation

ASJC Scopus subject areas

  • Immunology

Cite this

Tripathi, R., Jackson, A., & Krangel, M. S. (2002). A change in the structure of Vβ chromatin associated with TCR β allelic exclusion. Journal of Immunology, 168(5), 2316-2324.

A change in the structure of Vβ chromatin associated with TCR β allelic exclusion. / Tripathi, Rajkamal; Jackson, Annette; Krangel, Michael S.

In: Journal of Immunology, Vol. 168, No. 5, 01.03.2002, p. 2316-2324.

Research output: Contribution to journalArticle

Tripathi, R, Jackson, A & Krangel, MS 2002, 'A change in the structure of Vβ chromatin associated with TCR β allelic exclusion', Journal of Immunology, vol. 168, no. 5, pp. 2316-2324.
Tripathi, Rajkamal ; Jackson, Annette ; Krangel, Michael S. / A change in the structure of Vβ chromatin associated with TCR β allelic exclusion. In: Journal of Immunology. 2002 ; Vol. 168, No. 5. pp. 2316-2324.
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