A central nervous system specific mouse model for thanatophoric dysplasia type II

Ti Lin, Stacey B. Sandusky, Haipeng Xue, Kenneth W. Fishbein, Richard G. Spencer, Mahendra S. Rao, Clair A. Francomano

Research output: Contribution to journalArticle

Abstract

To investigate the specific effect of the Fgfr3 K644E mutation on central nervous system (CNS) development, we have generated tissue-specific TDII mice by crossing Fgfr3+/K644E-neo transgenic mice with CNS-specific Nestin-cre or cartilage-specific Col2a1-cre mice. TDII/Nestin-cre (TDII-N) neonates did not demonstrate a profound skeletal phenotype. TDII-N pups were comparable to their wild-type littermates in terms of tall length, fore and hindlimbs, and body weight; however, many pups exhibited notably round heads. MRI and histochemical analysis illustrated asymmetric changes in cortical thickness and cerebellar abnormalities in TDII-N mice, which correlate with brain abnormalities observed in human TDII patients. Such abnormalities were not seen in TDII/Col2a1-cre (TDII-C) mice. Upon examination of adult TDII-N spinal cord, premature differentiation of oligodendrocyte progenitors was observed. Overall, these data indicate that the tissue-specific mouse model is an excellent system for studying the role of Fgfr3 in the developing CNS.

Original languageEnglish (US)
Pages (from-to)2863-2871
Number of pages9
JournalHuman molecular genetics
Volume12
Issue number21
DOIs
StatePublished - Nov 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'A central nervous system specific mouse model for thanatophoric dysplasia type II'. Together they form a unique fingerprint.

Cite this