A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication

Katherine A. Jones, James T. Kadonaga, Philip J. Rosenfeld, Thomas Kelly, Robert Tjian

Research output: Contribution to journalArticle

Abstract

Transcription factor CTF, which is responsible for selective recognition of eukaryotic promoters that contain the sequence CCAAT, was purified to apparent homogeneity by sequence-specific DNA affinity chro-matography. Binding sites for CTF in the human Haras and α-globin promoters were highly homologous to sequences recognized by nuclear factor I (NF-I), a cellular DNA-binding protein that is required for the initiation of adenovirus DNA replication in vitro. To determine the relationship between CTF and NF-I, we compared the biochemical properties of these two proteins. CTF and NF-I were found to be indistinguishable in polypeptide composition, DNA-binding properties, immunological cross-reactivity, and in vitro stimulation of DNA replication and transcription initiation. We conclude that CTF/NF-I can serve both as a transcription selectivity factor for RNA polymerase II and as an initiation factor for adenovirus DNA replication.

Original languageEnglish (US)
Pages (from-to)79-89
Number of pages11
JournalCell
Volume48
Issue number1
DOIs
StatePublished - Jan 16 1987

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NFI Transcription Factors
DNA-Binding Proteins
Transcription
DNA Replication
DNA
Adenoviridae
Transcription Factors
Peptide Initiation Factors
Globins
RNA Polymerase II
Sequence Homology
Binding Sites
Peptides
Proteins
Chemical analysis
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication. / Jones, Katherine A.; Kadonaga, James T.; Rosenfeld, Philip J.; Kelly, Thomas; Tjian, Robert.

In: Cell, Vol. 48, No. 1, 16.01.1987, p. 79-89.

Research output: Contribution to journalArticle

Jones, Katherine A. ; Kadonaga, James T. ; Rosenfeld, Philip J. ; Kelly, Thomas ; Tjian, Robert. / A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication. In: Cell. 1987 ; Vol. 48, No. 1. pp. 79-89.
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