A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication

Katherine A. Jones, James T. Kadonaga, Philip J. Rosenfeld, Thomas J. Kelly, Robert Tjian

Research output: Contribution to journalArticle

Abstract

Transcription factor CTF, which is responsible for selective recognition of eukaryotic promoters that contain the sequence CCAAT, was purified to apparent homogeneity by sequence-specific DNA affinity chro-matography. Binding sites for CTF in the human Haras and α-globin promoters were highly homologous to sequences recognized by nuclear factor I (NF-I), a cellular DNA-binding protein that is required for the initiation of adenovirus DNA replication in vitro. To determine the relationship between CTF and NF-I, we compared the biochemical properties of these two proteins. CTF and NF-I were found to be indistinguishable in polypeptide composition, DNA-binding properties, immunological cross-reactivity, and in vitro stimulation of DNA replication and transcription initiation. We conclude that CTF/NF-I can serve both as a transcription selectivity factor for RNA polymerase II and as an initiation factor for adenovirus DNA replication.

Original languageEnglish (US)
Pages (from-to)79-89
Number of pages11
JournalCell
Volume48
Issue number1
DOIs
StatePublished - Jan 16 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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