A CD34+ human cell line model of myeloid dendritic cell differentiation: Evidence for a CD14+CD11b+ Langerhans cell precursor

Saskia J A M Santegoets, Allan J. Masterson, Pieter C. Van Der Sluis, Sinéad M. Lougheed, Donna M. Fluitsma, Alfons J M Van Den Eertwegh, Herbert M. Pinedo, Rik J. Scheper, Tanja D. De Gruijl

Research output: Contribution to journalArticlepeer-review

Abstract

The study of early events in dendritic cell (DC) differentiation is hampered by the lack of homogeneous primary cell systems that allow the study of cytokine-driven, transitional DC differentiation steps. The CD34+ acute myeloid leukemia cell line MUTZ-3 displays a unique ability to differentiate into interstitial DC (IDC) and Langerhans cells (LC) in a cytokine-dependent manner. Phenotypic characterization revealed MUTZ-3 to consist of three distinct subpopulations. Small CD34+CD14 -CD11b- progenitors constitute the proliferative compartment of the cell line with the ability to differentiate through a CD34-CD14- CD11b+ stage to ultimately give rise to a morphologically large, nonproliferating CD14+CD11bhi progeny. These CD14+CD11bhi cells were identified as common, immediate myeloid DC precursors with the ability to differentiate into LC and IDC, exhibiting characteristic and mutually exclusive expression of Langerin and DC-specific ICAM-grabbing nonintegrin, respectively. The identity of the MUTZ-3-derived LC subset was confirmed further by the presence of Birbeck granules. We conclude that the MUTZ-3 cell line provides a ready and continuous supply of common myeloid precursors, which should facilitate further study of the ontogeny of myeloid DC lineages.

Original languageEnglish (US)
Pages (from-to)1337-1344
Number of pages8
JournalJournal of Leukocyte Biology
Volume80
Issue number6
DOIs
StatePublished - Dec 2006
Externally publishedYes

Keywords

  • Acute myeloid leukemia
  • Cytokines
  • MUTZ-3
  • Progenitor

ASJC Scopus subject areas

  • Cell Biology

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