TY - JOUR
T1 - A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR
AU - Hardick, Justin
AU - Sadiq, Sufyan
AU - Perelstein, Elizabeth
AU - Peterson, Stephen
AU - Rothman, Richard
AU - Gaydos, Charlotte A.
N1 - Funding Information:
Funding: HHS PACER Project Domain/Number: 2010-ST_061 PA0001 grant 108822.
PY - 2014/6
Y1 - 2014/6
N2 - Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.
AB - Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.
KW - DFA
KW - RT-PCR/ESI-MS
KW - Respiratory virus diagnostics
KW - XTAG RVP
UR - http://www.scopus.com/inward/record.url?scp=84900471298&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84900471298&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2014.02.009
DO - 10.1016/j.diagmicrobio.2014.02.009
M3 - Article
C2 - 24657170
AN - SCOPUS:84900471298
SN - 0732-8893
VL - 79
SP - 187
EP - 189
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 2
ER -