A C. elegans-based, whole animal, in vivo screen for the identification of antifungal compounds.

Emmanouil Tampakakis, Ikechukwu Okoli, Eleftherios Mylonakis

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Traditional antimicrobial screens focus on compounds that block the growth of microbial organisms. A new Caenorhabditis elegans-based bioassay can be used for the identification of antifungal compounds that are effective against Candida albicans. According to the protocol, adult nematodes are infected with C. albicans and moved to 96-well plates containing the tested compounds. In the presence of compounds with no antifungal activity, the fungus kills the worms and develops filaments that penetrate through the cuticle. In contrast to traditional screening methods and mammalian models, this facile, time-efficient and less costly assay allows the study of Candida cells in nonplanktonic form and may allow the concurrent evaluation of toxicity and antifungal activity and identify compounds that target virulence factors or modify host immune response. The screening assay takes about 5-6 d depending on the experimental design.

Original languageEnglish (US)
Pages (from-to)1925-1931
Number of pages7
JournalNature protocols
Volume3
Issue number12
DOIs
StatePublished - 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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