A bulge binding agent with novel wedge-shape topology for stimulation of DNA triplet repeat strand slippage synthesis

Liangliang Liu, Heyang Li, Long Yi, Xing Yang, Xin Wen, Zhen Xi

Research output: Contribution to journalArticlepeer-review

Abstract

Expansion of DNA repeat sequences is associated with many human genetic diseases. Bulged DNA structures have been implicated as intermediates in DNA slippage within the DNA repeat regions. Herein a bulge binding agent with novel wedge-shape topology of the aromatic moiety was designed and synthesized. The compound-bulge DNA interactions were characterized via UV melting experiments, circular dichroism and were quantitated by surface plasmon resonance with Kd of 41.5 μM. This compound showed remarkable stimulation for DNA triplet repeat strand slippage synthesis in vitro.

Original languageEnglish (US)
Pages (from-to)6184-6188
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume18
Issue number23
DOIs
StatePublished - Dec 1 2008

Keywords

  • Bulge DNA
  • Phenothiazine
  • Slippage synthesis
  • Surface plasmon resonance
  • Wedge-shaped structure

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'A bulge binding agent with novel wedge-shape topology for stimulation of DNA triplet repeat strand slippage synthesis'. Together they form a unique fingerprint.

Cite this