A break-apart fluorescence in situ hybridization assay for detecting RET translocations in papillary thyroid carcinoma

Frank Chen, Douglas P. Clark, Anita L. Hawkins, Laura A. Morsberger, Constance A. Griffin

Research output: Contribution to journalArticle

Abstract

At least 15 different translocations have been described activating RET in papillary thyroid carcinomas. A break-apart fluorescence in situ hybridization (FISH) assay should detect many translocations involving the RET gene without requiring knowledge of the partner gene. G-banding and spectral karyotyping was performed to further characterize the papillary carcinoma cell line TPC-1. BAC clones flanking the 5′ and 3′ regions of RET were labeled with SpectrumRed and biotin detected with avidin-AMCA (blue). In addition to the previously described chromosomal t(1;10;21), TPC-1 was found to have del(7)(q22q31) and der(8)t(8;8)(p21;q11.2). With the BAC probes, TPC-1 interphase nuclei showed the expected signal pattern of one paired red-blue signal as well as separated red and blue signals from the rearranged RET gene in 93% of cells. Interphase nuclei from normal human lymphocytes showed two paired red-blue signals in 97% of cells. TPC-1 cells were found to have the previously described chromosomal rearrangement that involves chromosome 10, with few other cytogenetically detectable genomic alterations. RET rearrangement can be detected by a break-apart BAC FISH probe set in the interphase nuclei of TPC-1 cells. This assay can potentially detect clinically relevant translocations involving RET.

Original languageEnglish (US)
Pages (from-to)128-134
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume178
Issue number2
DOIs
Publication statusPublished - Oct 15 2007

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ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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