TY - JOUR
T1 - A blind study of the polymerase chain reaction for the detection of Mycobacterium tuberculosis DNA
AU - Doucet-Populaire, F.
AU - Lalande, V.
AU - Carpentier, E.
AU - Bourgoin, A.
AU - Dailloux, M.
AU - Bollet, C.
AU - Vachée, A.
AU - Moinard, D.
AU - Texier-Maugein, J.
AU - Carbonnelle, B.
AU - Grosset, J.
PY - 1996
Y1 - 1996
N2 - Setting: Nine French laboratories routinely involved in mycobacterial work. Objective: To assess the detection of Mycobacterium tuberculosis in experimental samples by polymerase chain reaction (PCR) using the insertion sequence IS6110 as a target for deoxyribonucleic acid (DNA) amplification. Design: Nine laboratories participated in a blind study of the detection of M. tuberculosis by PCR in 20 coded samples containing either a definite number of M. tuberculosis complex (positive samples) or environmental mycobacteria (four samples) or no mycobacteria (five samples). Results: Five laboratories reported false-positive PCR results, with an average rate of 7%. All laboratories except one reported positive PCR results for samples containing 105 cfu/ml or more, M. tuberculosis DNA was detected in two thirds of samples containing 104 and 103 cfu/ml, and in one third of the samples containing 102 cfu/ml. Conclusion: The results of the study suggest that PCR using IS6110 as a target for DNA amplication is neither very sensitive nor really specific for the detection of M. tuberculosis.
AB - Setting: Nine French laboratories routinely involved in mycobacterial work. Objective: To assess the detection of Mycobacterium tuberculosis in experimental samples by polymerase chain reaction (PCR) using the insertion sequence IS6110 as a target for deoxyribonucleic acid (DNA) amplification. Design: Nine laboratories participated in a blind study of the detection of M. tuberculosis by PCR in 20 coded samples containing either a definite number of M. tuberculosis complex (positive samples) or environmental mycobacteria (four samples) or no mycobacteria (five samples). Results: Five laboratories reported false-positive PCR results, with an average rate of 7%. All laboratories except one reported positive PCR results for samples containing 105 cfu/ml or more, M. tuberculosis DNA was detected in two thirds of samples containing 104 and 103 cfu/ml, and in one third of the samples containing 102 cfu/ml. Conclusion: The results of the study suggest that PCR using IS6110 as a target for DNA amplication is neither very sensitive nor really specific for the detection of M. tuberculosis.
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U2 - 10.1016/S0962-8479(96)90102-1
DO - 10.1016/S0962-8479(96)90102-1
M3 - Article
C2 - 8796253
AN - SCOPUS:0007998009
SN - 0962-8479
VL - 77
SP - 358
EP - 362
JO - Tubercle and Lung Disease
JF - Tubercle and Lung Disease
IS - 4
ER -