A blind study of the polymerase chain reaction for the detection of Mycobacterium tuberculosis DNA

F. Doucet-Populaire, V. Lalande, E. Carpentier, A. Bourgoin, M. Dailloux, C. Bollet, A. Vachée, D. Moinard, J. Texier-Maugein, B. Carbonnelle, J. Grosset

Research output: Contribution to journalArticle

Abstract

Setting: Nine French laboratories routinely involved in mycobacterial work. Objective: To assess the detection of Mycobacterium tuberculosis in experimental samples by polymerase chain reaction (PCR) using the insertion sequence IS6110 as a target for deoxyribonucleic acid (DNA) amplification. Design: Nine laboratories participated in a blind study of the detection of M. tuberculosis by PCR in 20 coded samples containing either a definite number of M. tuberculosis complex (positive samples) or environmental mycobacteria (four samples) or no mycobacteria (five samples). Results: Five laboratories reported false-positive PCR results, with an average rate of 7%. All laboratories except one reported positive PCR results for samples containing 105 cfu/ml or more, M. tuberculosis DNA was detected in two thirds of samples containing 104 and 103 cfu/ml, and in one third of the samples containing 102 cfu/ml. Conclusion: The results of the study suggest that PCR using IS6110 as a target for DNA amplication is neither very sensitive nor really specific for the detection of M. tuberculosis.

Original languageEnglish (US)
Pages (from-to)358-362
Number of pages5
JournalTubercle and Lung Disease
Volume77
Issue number4
DOIs
StatePublished - 1996
Externally publishedYes

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Mycobacterium tuberculosis
Polymerase Chain Reaction
DNA
Mycobacterium
Insertional Mutagenesis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Immunology
  • Microbiology

Cite this

Doucet-Populaire, F., Lalande, V., Carpentier, E., Bourgoin, A., Dailloux, M., Bollet, C., ... Grosset, J. (1996). A blind study of the polymerase chain reaction for the detection of Mycobacterium tuberculosis DNA. Tubercle and Lung Disease, 77(4), 358-362. https://doi.org/10.1016/S0962-8479(96)90102-1

A blind study of the polymerase chain reaction for the detection of Mycobacterium tuberculosis DNA. / Doucet-Populaire, F.; Lalande, V.; Carpentier, E.; Bourgoin, A.; Dailloux, M.; Bollet, C.; Vachée, A.; Moinard, D.; Texier-Maugein, J.; Carbonnelle, B.; Grosset, J.

In: Tubercle and Lung Disease, Vol. 77, No. 4, 1996, p. 358-362.

Research output: Contribution to journalArticle

Doucet-Populaire, F, Lalande, V, Carpentier, E, Bourgoin, A, Dailloux, M, Bollet, C, Vachée, A, Moinard, D, Texier-Maugein, J, Carbonnelle, B & Grosset, J 1996, 'A blind study of the polymerase chain reaction for the detection of Mycobacterium tuberculosis DNA', Tubercle and Lung Disease, vol. 77, no. 4, pp. 358-362. https://doi.org/10.1016/S0962-8479(96)90102-1
Doucet-Populaire, F. ; Lalande, V. ; Carpentier, E. ; Bourgoin, A. ; Dailloux, M. ; Bollet, C. ; Vachée, A. ; Moinard, D. ; Texier-Maugein, J. ; Carbonnelle, B. ; Grosset, J. / A blind study of the polymerase chain reaction for the detection of Mycobacterium tuberculosis DNA. In: Tubercle and Lung Disease. 1996 ; Vol. 77, No. 4. pp. 358-362.
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