A biosensor based on micromechanical interrogation of living cells

Matthew D. Antonik; Neill P. D'Costa; Jan H. Hoh

doi:10.1109/51.582178

A biosensor based on micromechanical interrogation of living cells

Matthew D. Antonik, Neill P. D'Costa, Jan H. Hoh

Research output: Contribution to journal › Review article › peer-review

38 Scopus citations

Abstract

Living cells can be reliably cultured directly onto AFM cantilevers. The cells are inclined to grow on both sides of the surface, and the cantilevers must be treated to deter such two-sided growth. Preliminary data acquired with the AFM demonstrated that these integrated cells/cantilevers are capable of detecting the response of MDCK cells to different toxins, with response time on the order of several seconds. These results suggest the feasibility of real-time, rapid, and sensitive biomechanical sensors. The initial temperature results from the fluid chamber prototype indicate the potential for a low-cost dedicated system.

Original language	English (US)
Pages (from-to)	66-72
Number of pages	7
Journal	IEEE Engineering in Medicine and Biology Magazine
Volume	16
Issue number	2
DOIs	https://doi.org/10.1109/51.582178
State	Published - Mar 1997
Externally published	Yes

ASJC Scopus subject areas

Biomedical Engineering

Access to Document

10.1109/51.582178

Cite this

@article{98ff42a0c9f64fdbb3c7db69be2fd248,

title = "A biosensor based on micromechanical interrogation of living cells",

abstract = "Living cells can be reliably cultured directly onto AFM cantilevers. The cells are inclined to grow on both sides of the surface, and the cantilevers must be treated to deter such two-sided growth. Preliminary data acquired with the AFM demonstrated that these integrated cells/cantilevers are capable of detecting the response of MDCK cells to different toxins, with response time on the order of several seconds. These results suggest the feasibility of real-time, rapid, and sensitive biomechanical sensors. The initial temperature results from the fluid chamber prototype indicate the potential for a low-cost dedicated system.",

author = "Antonik, {Matthew D.} and D'Costa, {Neill P.} and Hoh, {Jan H.}",

note = "Funding Information: We would like to acknowledge Henry Brown{\textquoteright}s help in the thin section analysis of the webbed cantilevers This work was supported by grants from the Muscular Dystrophy Association and the Whitaker Foundation to Jan Hoh",

year = "1997",

month = mar,

doi = "10.1109/51.582178",

language = "English (US)",

volume = "16",

pages = "66--72",

journal = "IEEE Engineering in Medicine and Biology Magazine",

issn = "0739-5175",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

number = "2",

}

TY - JOUR

T1 - A biosensor based on micromechanical interrogation of living cells

AU - Antonik, Matthew D.

AU - D'Costa, Neill P.

AU - Hoh, Jan H.

N1 - Funding Information: We would like to acknowledge Henry Brown’s help in the thin section analysis of the webbed cantilevers This work was supported by grants from the Muscular Dystrophy Association and the Whitaker Foundation to Jan Hoh

PY - 1997/3

Y1 - 1997/3

N2 - Living cells can be reliably cultured directly onto AFM cantilevers. The cells are inclined to grow on both sides of the surface, and the cantilevers must be treated to deter such two-sided growth. Preliminary data acquired with the AFM demonstrated that these integrated cells/cantilevers are capable of detecting the response of MDCK cells to different toxins, with response time on the order of several seconds. These results suggest the feasibility of real-time, rapid, and sensitive biomechanical sensors. The initial temperature results from the fluid chamber prototype indicate the potential for a low-cost dedicated system.

AB - Living cells can be reliably cultured directly onto AFM cantilevers. The cells are inclined to grow on both sides of the surface, and the cantilevers must be treated to deter such two-sided growth. Preliminary data acquired with the AFM demonstrated that these integrated cells/cantilevers are capable of detecting the response of MDCK cells to different toxins, with response time on the order of several seconds. These results suggest the feasibility of real-time, rapid, and sensitive biomechanical sensors. The initial temperature results from the fluid chamber prototype indicate the potential for a low-cost dedicated system.

UR - http://www.scopus.com/inward/record.url?scp=0031104805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031104805&partnerID=8YFLogxK

U2 - 10.1109/51.582178

DO - 10.1109/51.582178

M3 - Review article

C2 - 9086374

AN - SCOPUS:0031104805

SN - 0739-5175

VL - 16

SP - 66

EP - 72

JO - IEEE Engineering in Medicine and Biology Magazine

JF - IEEE Engineering in Medicine and Biology Magazine

IS - 2

ER -

A biosensor based on micromechanical interrogation of living cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this