A biopsy-needle compatible varifocal multiphoton rigid probe for depth-resolved optical biopsy

Ang Li, Gunnsteinn Hall, Defu Chen, Wenxuan Liang, Bo Ning, Honghua Guan, Xingde Li

Research output: Contribution to journalArticle

Abstract

In this work, we report a biopsy-needle compatible rigid probe, capable of performing three-dimensional (3D) two-photon optical biopsy. The probe has a small outer diameter of 1.75 mm and fits inside a gauge-14 biopsy needle to reach internal organs. A carefully designed focus scanning mechanism has been implemented in the rigid probe, which, along with a rapid two-dimensional MEMS scanner, enables 3D imaging. Fast image acquisition up to 10 frames per second is possible, dramatically reducing motion artifacts during in vivo imaging. Equipped with a high-numerical aperture micro-objective, the miniature rigid probe offers a high two-photon resolution (0.833 × 6.11 μm, lateral × axial), a lateral field of view of 120 μm, and an axial focus tuning range of 200 μm. In addition to imaging of mouse internal organs and subcutaneous tumor in vivo, first-of-its-kind depth-resolved two-photon optical biopsy of an internal organ has been successfully demonstrated on mouse kidney in vivo and in situ.

Original languageEnglish (US)
JournalJournal of Biophotonics
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Biopsy
Needle Biopsy
Photons
needles
Needles
optical thickness
organs
probes
Imaging techniques
Micro-Electrical-Mechanical Systems
mice
photons
Artifacts
Image acquisition
kidneys
numerical aperture
scanners
Gages
microelectromechanical systems
MEMS

Keywords

  • depth-resolved
  • endoscope
  • label-free
  • optical biopsy
  • rigid probe
  • two-photon microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

A biopsy-needle compatible varifocal multiphoton rigid probe for depth-resolved optical biopsy. / Li, Ang; Hall, Gunnsteinn; Chen, Defu; Liang, Wenxuan; Ning, Bo; Guan, Honghua; Li, Xingde.

In: Journal of Biophotonics, 01.01.2018.

Research output: Contribution to journalArticle

Li, Ang ; Hall, Gunnsteinn ; Chen, Defu ; Liang, Wenxuan ; Ning, Bo ; Guan, Honghua ; Li, Xingde. / A biopsy-needle compatible varifocal multiphoton rigid probe for depth-resolved optical biopsy. In: Journal of Biophotonics. 2018.
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