A bioorthogonal 68Ga-labelling strategy for rapid in vivo imaging

Helen L. Evans; Quang Dé Nguyen; Laurence S. Carroll; Maciej Kaliszczak; Frazer J. Twyman; Alan C. Spivey; Eric O. Aboagye

doi:10.1039/c4cc03903c

A bioorthogonal ⁶⁸Ga-labelling strategy for rapid in vivo imaging

Helen L. Evans, Quang Dé Nguyen, Laurence S. Carroll, Maciej Kaliszczak, Frazer J. Twyman, Alan C. Spivey, Eric O. Aboagye

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Herein, we describe a fast and robust method for achieving ⁶⁸Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels–Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.

Original language	English (US)
Pages (from-to)	9557-9560
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	67
DOIs	https://doi.org/10.1039/c4cc03903c
State	Published - Jul 24 2014
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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abstract = "Herein, we describe a fast and robust method for achieving 68Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels–Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.",

author = "Evans, {Helen L.} and Nguyen, {Quang D{\'e}} and Carroll, {Laurence S.} and Maciej Kaliszczak and Twyman, {Frazer J.} and Spivey, {Alan C.} and Aboagye, {Eric O.}",

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AU - Evans, Helen L.

AU - Nguyen, Quang Dé

AU - Carroll, Laurence S.

AU - Kaliszczak, Maciej

AU - Twyman, Frazer J.

AU - Spivey, Alan C.

AU - Aboagye, Eric O.

PY - 2014/7/24

Y1 - 2014/7/24

N2 - Herein, we describe a fast and robust method for achieving 68Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels–Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.

AB - Herein, we describe a fast and robust method for achieving 68Ga-labelling of the EGFR-selective monoclonal antibody (mAb) Cetuximab using the bioorthogonal Inverse-electron-Demand Diels–Alder (IeDDA) reaction. The in vivo imaging of EGFR is demonstrated, as well as the translation of the method within a two-step pretargeting strategy.

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A bioorthogonal ⁶⁸Ga-labelling strategy for rapid in vivo imaging

Abstract

ASJC Scopus subject areas

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