@article{5907155c9b1548f2a4982188bcb06464,
title = "A Biomimetic Phosphatidylcholine-Terminated Monolayer Greatly Improves the In Vivo Performance of Electrochemical Aptamer-Based Sensors",
abstract = "The real-time monitoring of specific analytes in situ in the living body would greatly advance our understanding of physiology and the development of personalized medicine. Because they are continuous (wash-free and reagentless) and are able to work in complex media (e.g., undiluted serum), electrochemical aptamer-based (E-AB) sensors are promising candidates to fill this role. E-AB sensors suffer, however, from often-severe baseline drift when deployed in undiluted whole blood either in vitro or in vivo. We demonstrate that cell-membrane-mimicking phosphatidylcholine (PC)-terminated monolayers improve the performance of E-AB sensors, reducing the baseline drift from around 70 % to just a few percent after several hours in flowing whole blood in vitro. With this improvement comes the ability to deploy E-AB sensors directly in situ in the veins of live animals, achieving micromolar precision over many hours without the use of physical barriers or active drift-correction algorithms.",
keywords = "aptamers, biomimetic surfaces, electrochemical sensors, in vivo measurements, membrane monolayers",
author = "Hui Li and Philippe Dauphin-Ducharme and Netzahualc{\'o}yotl Arroyo-Curr{\'a}s and Tran, {Claire H.} and Vieira, {Philip A.} and Shaoguang Li and Christina Shin and Jacob Somerson and Kippin, {Tod E.} and Plaxco, {Kevin W.}",
note = "Funding Information: The biosensor work at UCSB was carried out at the Institute for Collaborative Biotechnologies (supported by the Army Research Office, Grant W911NF-09-0001 and the National Institutes of Health, Grant R01AI107936) and with partial support from the W. M. Keck Foundation. L. H. was partially supported by Swiss National Science Foundation with an “Early Postdoc Mobility fellowship”. P. D.-D. is partially supported by Fonds de recherche du Quebec—Nature et Technologies and Natural Sciences and Engineering Research Council with a postdoctoral fellowship. N. A.-C. is supported by the Otis Williams Postdoctoral Fellowship of the Santa Barbara foundation. J. S. is supported by the National Cancer Institute of the National Institutes of Health (NRSA F31CA183385). The authors thank Dr. Gabriel Ortega Quintanilla for help with Matlab script for data processing. Funding Information: The biosensor work at UCSB was carried out at the Institute for Collaborative Biotechnologies (supported by the Army Research Office, Grant W911NF-09-0001 and the National Institutes of Health, Grant R01AI107936) and with partial support from the W. M. Keck Foundation. L. H. was partially supported by Swiss National Science Foundation with an ?Early Postdoc Mobility fellowship?. P. D.-D. is partially supported by Fonds de recherche du Quebec?Nature et Technologies and Natural Sciences and Engineering Research Council with a postdoctoral fellowship. N. A.-C. is supported by the Otis Williams Postdoctoral Fellowship of the Santa Barbara foundation. J. S. is supported by the National Cancer Institute of the National Institutes of Health (NRSA F31CA183385). The authors thank Dr. Gabriel Ortega Quintanilla for help with Matlab script for data processing. Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2017",
month = jun,
day = "19",
doi = "10.1002/anie.201700748",
language = "English (US)",
volume = "56",
pages = "7492--7495",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "26",
}