A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome

Raymond R. Samaha, Rachel Green, Harry F. Noller

Research output: Contribution to journalArticle

Abstract

Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A Watson-Crick G-C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.

Original languageEnglish (US)
Pages (from-to)309-314
Number of pages6
JournalNature
Volume377
Issue number6547
StatePublished - 1995
Externally publishedYes

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Peptidyl Transferases
Transfer RNA
Ribosomes
Base Pairing
Proteins

ASJC Scopus subject areas

  • General

Cite this

A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome. / Samaha, Raymond R.; Green, Rachel; Noller, Harry F.

In: Nature, Vol. 377, No. 6547, 1995, p. 309-314.

Research output: Contribution to journalArticle

Samaha, Raymond R. ; Green, Rachel ; Noller, Harry F. / A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome. In: Nature. 1995 ; Vol. 377, No. 6547. pp. 309-314.
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