A bacterial/yeast merged two-hybrid system

protocol for yeast screening with single or parallel baits.

Nadezhda Y. Tikhmyanova, Evgeny Izumchenko, Ilya G. Serebriiskii, Erica A. Golemis

Research output: Contribution to journalArticle

Abstract

The yeast two-hybrid system is a useful tool for identifying new protein-protein interactions, and for the dissection of previously identified interactions. An important issue in protein-interaction studies is frequently that of determining whether a protein associates specifically with one protein or domain of interest, or has a more promiscuous interaction profile. To help address this issue, the authors have created a new two-hybrid system, which can be used either in bacteria or in yeast to counterscreen against "decoy" baits in parallel with a primary screen, hence improving the power and specificity of the method. Protocols of this system for use in yeast are provided; a companion article, Serebriiski et al., describes alternative use of this system in bacteria.

Original languageEnglish (US)
Pages (from-to)257-290
Number of pages34
JournalMethods in molecular biology (Clifton, N.J.)
Volume408
StatePublished - 2007
Externally publishedYes

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Two-Hybrid System Techniques
Yeasts
Proteins
Bacteria
Dissection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Tikhmyanova, N. Y., Izumchenko, E., Serebriiskii, I. G., & Golemis, E. A. (2007). A bacterial/yeast merged two-hybrid system: protocol for yeast screening with single or parallel baits. Methods in molecular biology (Clifton, N.J.), 408, 257-290.

A bacterial/yeast merged two-hybrid system : protocol for yeast screening with single or parallel baits. / Tikhmyanova, Nadezhda Y.; Izumchenko, Evgeny; Serebriiskii, Ilya G.; Golemis, Erica A.

In: Methods in molecular biology (Clifton, N.J.), Vol. 408, 2007, p. 257-290.

Research output: Contribution to journalArticle

Tikhmyanova, Nadezhda Y. ; Izumchenko, Evgeny ; Serebriiskii, Ilya G. ; Golemis, Erica A. / A bacterial/yeast merged two-hybrid system : protocol for yeast screening with single or parallel baits. In: Methods in molecular biology (Clifton, N.J.). 2007 ; Vol. 408. pp. 257-290.
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