A β2-adrenergic agonist inhibits dry air-induced injury in canine peripheral airways

C. Omori, B. H. Schofield, W. Mitzner, A. N. Freed

Research output: Contribution to journalArticlepeer-review

Abstract

We examined the effects of β2-agonist on dry air-induced injury in canine peripheral airways. Dry air-induced bronchoconstriction (AIB) was assessed by measuring peripheral airway resistance in anesthetized dogs. Salbutamol reduced AIB by ~75% compared with control values. Colloidal carbon was used to detect bronchovascular leakage in contralateral sublobar segments that were pretreated with saline or salbutamol. About 87% of the perimeter of bronchi was damaged after dry air challenge in saline-treated segments. Salbutamol reduced mucosal damage by ~30% (P < 0.05). The mucosa of bronchioles was not injured. The average goblet-to-ciliated cell ratio (which reflects mucosal perturbation) in bronchi decreased from 0.38 in control bronchi to 0.15 in challenged bronchi, and this effect was also evident in bronchioles. Salbutamol did not affect this decrement. Dry air challenge also caused degranulation of mast cells located below damaged mucosa, dilation of bronchial vessels, and leakage from capillaries and venules located below normal ciliated and damaged mucosa of bronchi. Thus, we conclude that salbutamol attenuates epithelial damage and AIB but fails to inhibit mast cell degranulation and vascular hyperpermeability.

Original languageEnglish (US)
Pages (from-to)2169-2179
Number of pages11
JournalJournal of applied physiology
Volume78
Issue number6
DOIs
StatePublished - 1995

Keywords

  • asthma
  • bronchial circulation
  • bronchovascular leakage
  • lung injury
  • mast cells
  • β-adrenoreceptor agonist

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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