Aβ(25-35) and Aβ(1-40) act on different calcium channels in CA1 hippocampal neurons

Catherine Rovira, Nicolas Arbez, Jean Mariani

Research output: Contribution to journalArticlepeer-review

Abstract

The acute effects of β-amyloid (25-35) and (1-40) on high voltage activated calcium channels were compared in CA1 pyramidal cells of adult mouse hippocampal slices using the whole-cell patch-clamp recording. Bath application of oligomeric β-amyloid (25-35) reversibly increased the barium current (IBa) to 1.61 (normalized amplitude), while oligomeric β-amyloid (1-40) reversibly enhanced the IBa to 1.74. Reverse-sequence β-amyloid [(35-25) and (40-1)] had no effect. The effect of β-amyloid (25-35) was blocked by nifedipine, a selective antagonist of L-type calcium channels. In contrast, the effect of β-amyloid (1-40) was not blocked by nifedipine and IBa was enhanced to 4.96. It is concluded that these oligomeric peptides may act through different types of calcium channels and/or receptors. The toxicity of Aβ(25-35) implicates a potentiation of L-type calcium channels while the one of Aβ(1-40) is related to an increase of non-L-type calcium channels, which may involve an increase in transmitter release.

Original languageEnglish (US)
Pages (from-to)1317-1321
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number5
DOIs
StatePublished - Oct 14 2002
Externally publishedYes

Keywords

  • Alzheimer
  • Calcium channels
  • Hippocampus
  • β-Amyloid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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