Aβ(25-35) and Aβ(1-40) act on different calcium channels in CA1 hippocampal neurons

Catherine Rovira; Nicolas Arbez; Jean Mariani

doi:10.1016/S0006-291X(02)02072-7

Aβ(25-35) and Aβ(1-40) act on different calcium channels in CA1 hippocampal neurons

Catherine Rovira, Nicolas Arbez, Jean Mariani

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

The acute effects of β-amyloid (25-35) and (1-40) on high voltage activated calcium channels were compared in CA1 pyramidal cells of adult mouse hippocampal slices using the whole-cell patch-clamp recording. Bath application of oligomeric β-amyloid (25-35) reversibly increased the barium current (I_Ba) to 1.61 (normalized amplitude), while oligomeric β-amyloid (1-40) reversibly enhanced the I_Ba to 1.74. Reverse-sequence β-amyloid [(35-25) and (40-1)] had no effect. The effect of β-amyloid (25-35) was blocked by nifedipine, a selective antagonist of L-type calcium channels. In contrast, the effect of β-amyloid (1-40) was not blocked by nifedipine and I_Ba was enhanced to 4.96. It is concluded that these oligomeric peptides may act through different types of calcium channels and/or receptors. The toxicity of Aβ(25-35) implicates a potentiation of L-type calcium channels while the one of Aβ(1-40) is related to an increase of non-L-type calcium channels, which may involve an increase in transmitter release.

Original language	English (US)
Pages (from-to)	1317-1321
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	296
Issue number	5
DOIs	https://doi.org/10.1016/S0006-291X(02)02072-7
State	Published - 2002
Externally published	Yes

Keywords

Alzheimer
Calcium channels
Hippocampus
β-Amyloid

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(02)02072-7

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title = "Aβ(25-35) and Aβ(1-40) act on different calcium channels in CA1 hippocampal neurons",

abstract = "The acute effects of β-amyloid (25-35) and (1-40) on high voltage activated calcium channels were compared in CA1 pyramidal cells of adult mouse hippocampal slices using the whole-cell patch-clamp recording. Bath application of oligomeric β-amyloid (25-35) reversibly increased the barium current (IBa) to 1.61 (normalized amplitude), while oligomeric β-amyloid (1-40) reversibly enhanced the IBa to 1.74. Reverse-sequence β-amyloid [(35-25) and (40-1)] had no effect. The effect of β-amyloid (25-35) was blocked by nifedipine, a selective antagonist of L-type calcium channels. In contrast, the effect of β-amyloid (1-40) was not blocked by nifedipine and IBa was enhanced to 4.96. It is concluded that these oligomeric peptides may act through different types of calcium channels and/or receptors. The toxicity of Aβ(25-35) implicates a potentiation of L-type calcium channels while the one of Aβ(1-40) is related to an increase of non-L-type calcium channels, which may involve an increase in transmitter release.",

keywords = "Alzheimer, Calcium channels, Hippocampus, β-Amyloid",

author = "Catherine Rovira and Nicolas Arbez and Jean Mariani",

note = "Funding Information: We are grateful to Dr. Ann Lohof for critical reading of the paper. This work has been supported by a grant by Fondation pour la Recherche Medicale.",

year = "2002",

doi = "10.1016/S0006-291X(02)02072-7",

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T1 - Aβ(25-35) and Aβ(1-40) act on different calcium channels in CA1 hippocampal neurons

AU - Rovira, Catherine

AU - Arbez, Nicolas

AU - Mariani, Jean

N1 - Funding Information: We are grateful to Dr. Ann Lohof for critical reading of the paper. This work has been supported by a grant by Fondation pour la Recherche Medicale.

PY - 2002

Y1 - 2002

N2 - The acute effects of β-amyloid (25-35) and (1-40) on high voltage activated calcium channels were compared in CA1 pyramidal cells of adult mouse hippocampal slices using the whole-cell patch-clamp recording. Bath application of oligomeric β-amyloid (25-35) reversibly increased the barium current (IBa) to 1.61 (normalized amplitude), while oligomeric β-amyloid (1-40) reversibly enhanced the IBa to 1.74. Reverse-sequence β-amyloid [(35-25) and (40-1)] had no effect. The effect of β-amyloid (25-35) was blocked by nifedipine, a selective antagonist of L-type calcium channels. In contrast, the effect of β-amyloid (1-40) was not blocked by nifedipine and IBa was enhanced to 4.96. It is concluded that these oligomeric peptides may act through different types of calcium channels and/or receptors. The toxicity of Aβ(25-35) implicates a potentiation of L-type calcium channels while the one of Aβ(1-40) is related to an increase of non-L-type calcium channels, which may involve an increase in transmitter release.

AB - The acute effects of β-amyloid (25-35) and (1-40) on high voltage activated calcium channels were compared in CA1 pyramidal cells of adult mouse hippocampal slices using the whole-cell patch-clamp recording. Bath application of oligomeric β-amyloid (25-35) reversibly increased the barium current (IBa) to 1.61 (normalized amplitude), while oligomeric β-amyloid (1-40) reversibly enhanced the IBa to 1.74. Reverse-sequence β-amyloid [(35-25) and (40-1)] had no effect. The effect of β-amyloid (25-35) was blocked by nifedipine, a selective antagonist of L-type calcium channels. In contrast, the effect of β-amyloid (1-40) was not blocked by nifedipine and IBa was enhanced to 4.96. It is concluded that these oligomeric peptides may act through different types of calcium channels and/or receptors. The toxicity of Aβ(25-35) implicates a potentiation of L-type calcium channels while the one of Aβ(1-40) is related to an increase of non-L-type calcium channels, which may involve an increase in transmitter release.

KW - Alzheimer

KW - Calcium channels

KW - Hippocampus

KW - β-Amyloid

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Aβ(25-35) and Aβ(1-40) act on different calcium channels in CA1 hippocampal neurons

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Keywords

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