6‐Deoxy‐D‐glucose and D‐xylose: Analogs for the study of D‐glucose transport by mouse 3T3 cells

Antonio H. Romano, Nancy D. Connell

Research output: Contribution to journalArticle

Abstract

6‐Deoxy‐D‐glucose and D‐xylose, structural homomorphs of D‐glucose that lack a 6‐hydroxyl group or a 6‐hydroxymethyl group, respectively, are transported efficiently by mouse 3T3 cells, with good affinity and high specificity for the D‐glucose transport system. Since these analogs lack the 6‐hydroxyl group, which is the site of phosphorylation of glucose by hexokinase, they are taken up and are recoverable from cells in an unchanged state. Thus, 6‐deoxy‐D‐glucose and D‐xylose offer advantages as transport substrates over 2‐deoxy‐D‐glucose, which is phosphorylated by intercellular hexokinases, and 3‐O‐methyl‐D‐glucose, which shows a lower specificity for the D‐glucose transport system.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalJournal of Cellular Physiology
Volume111
Issue number1
DOIs
StatePublished - Apr 1982
Externally publishedYes

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ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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