6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition

Han Shin Kim, Sang Hoon Lee, Youngjoo Byun, Hee Deung Park

Research output: Contribution to journalArticle

Abstract

Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors via quorum sensing (QS). Interfering with normal QS interactions between signal molecules and their cognate receptors is a developing strategy for attenuating its virulence. Here we tested the hypothesis that 6-gingerol, a pungent oil of fresh ginger, reduces biofilm formation and virulence by antagonistically binding to P. aeruginosa QS receptors. In silico studies demonstrated molecular binding occurs between 6-gingerol and the QS receptor LasR through hydrogen bonding and hydrophobic interactions. Experimentally 6-gingerol reduced biofilm formation, several virulence factors (e.g., exoprotease, rhamnolipid, and pyocyanin), and mice mortality. Further transcriptome analyses demonstrated that 6-gingerol successfully repressed QS-induced genes, specifically those related to the production of virulence factors. These results strongly support our hypothesis and offer insight into the molecular mechanism that caused QS gene repression.

Original languageEnglish (US)
Article number8656
JournalScientific Reports
Volume5
DOIs
StatePublished - 2015
Externally publishedYes

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Quorum Sensing
Biofilms
Pseudomonas aeruginosa
Virulence
Virulence Factors
Pyocyanine
Exopeptidases
Ginger
Gene Expression Profiling
Hydrogen Bonding
Hydrophobic and Hydrophilic Interactions
Computer Simulation
Genes
gingerol
Oils
Bacteria
Mortality

ASJC Scopus subject areas

  • General

Cite this

6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition. / Kim, Han Shin; Lee, Sang Hoon; Byun, Youngjoo; Park, Hee Deung.

In: Scientific Reports, Vol. 5, 8656, 2015.

Research output: Contribution to journalArticle

Kim, Han Shin ; Lee, Sang Hoon ; Byun, Youngjoo ; Park, Hee Deung. / 6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition. In: Scientific Reports. 2015 ; Vol. 5.
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