53BP1 promotes microhomology-mediated end-joining in G1-phase cells

Xiahui Xiong, Zhanwen Du, Ying Wang, Zhihui Feng, Pan Fan, Chunhong Yan, Henning Willers, Junran Zhang

Research output: Research - peer-reviewArticle

Abstract

Alternative non-homologous end joining (alt-NHEJ) was originally identified as a backup repair mechanism in the absence of classical NHEJ (c-NHEJ) factors but recent studies have demonstrated that alt-NHEJ is active even when c-NHEJ as well as homologous recombination is available. The functions of 53BP1 in NHEJ processes are not well understood. Here, we report that 53BP1 promotes DNA double-strand break (DSB) repair and genomic stability not only in c-NHEJ-proficient but also -deficient human G1-phase cells. Using an array of repair substrates we show that these effects of 53BP1 are correlated with a promotion of microhomology-mediated end-joining (MMEJ), a subtype of alt-NHEJ, in G1-phase. Consistent with a specific role in MMEJ we confirm that 53BP1 status does not affect c-NHEJ. 53BP1 supports sequence deletion during MMEJ consistent with a putative role in facilitating end-resection. Interestingly, promotion of MMEJ by 53BP1 in G1-phase cells is only observed in the presence of functional BRCA1. Depletion of both 53BP1 and BRCA1 increases repair needing microhomology usage and augments loss of DNA sequence, suggesting that MMEJ is a highly regulated DSB repair process. Together, these findings significantly expand our understanding of the cell-cycle-dependent roles of 53BP1 in DSB repair.

LanguageEnglish (US)
Pages1659-1670
Number of pages12
JournalNucleic Acids Research
Volume43
Issue number3
DOIs
StatePublished - 2015

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G1 Phase
Double-Stranded DNA Breaks
Sequence Deletion
Genomic Instability
Homologous Recombination
Cell Cycle

ASJC Scopus subject areas

  • Genetics

Cite this

Xiong, X., Du, Z., Wang, Y., Feng, Z., Fan, P., Yan, C., ... Zhang, J. (2015). 53BP1 promotes microhomology-mediated end-joining in G1-phase cells. Nucleic Acids Research, 43(3), 1659-1670. DOI: 10.1093/nar/gku1406

53BP1 promotes microhomology-mediated end-joining in G1-phase cells. / Xiong, Xiahui; Du, Zhanwen; Wang, Ying; Feng, Zhihui; Fan, Pan; Yan, Chunhong; Willers, Henning; Zhang, Junran.

In: Nucleic Acids Research, Vol. 43, No. 3, 2015, p. 1659-1670.

Research output: Research - peer-reviewArticle

Xiong, X, Du, Z, Wang, Y, Feng, Z, Fan, P, Yan, C, Willers, H & Zhang, J 2015, '53BP1 promotes microhomology-mediated end-joining in G1-phase cells' Nucleic Acids Research, vol 43, no. 3, pp. 1659-1670. DOI: 10.1093/nar/gku1406
Xiong X, Du Z, Wang Y, Feng Z, Fan P, Yan C et al. 53BP1 promotes microhomology-mediated end-joining in G1-phase cells. Nucleic Acids Research. 2015;43(3):1659-1670. Available from, DOI: 10.1093/nar/gku1406
Xiong, Xiahui ; Du, Zhanwen ; Wang, Ying ; Feng, Zhihui ; Fan, Pan ; Yan, Chunhong ; Willers, Henning ; Zhang, Junran. / 53BP1 promotes microhomology-mediated end-joining in G1-phase cells. In: Nucleic Acids Research. 2015 ; Vol. 43, No. 3. pp. 1659-1670
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